MAR 28, 2018 10:00 AM PDT

Primary Cell Systems: Offerings and Applications from basic biology and tissue modeling to compound screening

SPONSORED BY: Thermo Fisher Scientific
6 20 2263

  • Associate Director and Group Leader, Thermo Fisher Scientific
      Dr. Kuninger leads research, development and commercialization of media systems for pluripotent stem cell culture & differentiation, neurobiology, and (non-hepatic) primary cell biology at Thermo Fisher Scientific in the Cell Biology business based in Frederick MD. His teams support numerous portfolios and have launched over 25 new products spanning stem cell culture & cryopreservation, differentiation (endo-, ecto- and meso-dermal lineages) and neurobiology over the past 3 years. David is a seasoned scientist and manager, experienced in media formulation & optimization, assay design and implementation, and troubleshooting. Expertise in GLP/GMP compliance, tech transfer and scale up, as well as verification and validation processes. Prior to starting at Thermo Fisher Scientific (legacy Invitrogen) in 2007 as Staff Scientist, he joined Oregon Health Sciences University as a Postdoctoral Fellow investigating the actions of insulin-like growth factors in the lab of Dr. Peter Rotwein, subsequently joining the faculty in the Department of Biochemistry at OHSU as a Research Instructor. He completed is PhD in Biochemistry and Genetics University of Texas Medical Branch in the laboratory of Dr. John Papaconstatinou and has a B.S. in Chemistry from the University of Oregon.
    • Senior Staff Scientist, Cell Biology, Thermo Fisher Scientific
        Dr. Newman joined the Primary and Stem Cell Systems Division Life Technologies in 2009 and initially focused on isolation and expansion of human primary cells for development of 3D organotypic models. Currently, Dr. Newman continues to support primary cell efforts and works on development of next generation stem cell culture and differentiation systems, enabling researchers to efficiently culture, expand, cryopreserve, and differentiate their stem cells to various cellular lineages. She received a Ph.D. at the University of Iowa from the lab of Dr. Madeline Shea, focusing on ligand induced allostery of calmodulin and its impact on regulation of the Ryanodine Receptor Type 1. Subsequently, she completed postdoctoral training in the lab of Dr. Ken Prehoda at the University of Oregon, studying the role of intramolecular interactions in regulating cell signaling cascades in the process of assymetric stem cell division.


      DATE: March 28, 2018
      TIME: 10:00am PDT, 01:00pm EDT

      Primary cells are derived from various tissues typically via dissection, enzymatic dissociation, and subsequent culture in media specifically designed to support expansion of defined cell types.   Following expansion, primary cells largely retain the characteristics of the tissue from which they were isolated, providing greater physiological relevance relative to immortalized cell lines.  Thermo Fisher Scientific offers a wide array of primary cells from both human and non-human sources and specialized culture supports, including media, matrices, and passaging reagents.  In this webinar we will review the scope of our primary cells and culture reagents and discuss factors for consideration when deciding between primary cell and stem cell-derived models.  We will present examples of incorporating specific pathway sensors into primary cells to create reporters in a normal adult human background.  In addition, we will highlight the use of primary cells for creating 3D organotypic epithelial models.  The incorporation of primary human cells into 3D tissue models enables the in vitro recapitulation of complex human physiology, providing sophisticated biological models for research and product development.    Finally, we will share our perspective on how primary cell models will continue to complement advances in stem cell biology.

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