FEB 08, 2017 06:00 AM PST

Producing Petri Puppies: Gamete Collection and In Vitro Fertilization in the Domestic Dog

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  • Postdoctoral Fellow, Smithsonian Conservation Biology Institute
      Jennifer Nagashima is a Smithsonian Institution postdoctoral fellow for the Smithsonian Conservation Biology Institute (SCBI) with Nucharin Songsasen. Her current research focuses on developing new "gamete rescue" technologies in canids and felids, include freezing sperm and culturing ovaries in vitro to grow mature eggs.

      Nagashima earned her bachelor's degree from Cornell University in animal science in 2009 and completed her doctorate in zoology and wildlife conservation in 2015. As part of her doctoral dissertation in the Joint Graduate Training Program (JGTP) between Cornell University and SCBI, Nagashima produced the world's first litter of puppies from in vitro fertilization and cultured domestic dog ovarian follicles using cutting-edge, three-dimensional systems, with the goal of applying these technologies to the conservation of wild canids in the future.


    DATE: February 8, 2017
    TIME: 6:00am PT, 9:00am ET

    Development of assisted reproductive technologies in the dog has lagged behind many other domestic and laboratory animal species, due in part to their unique reproductive biology. Here, we will discuss the challenges with dog ART and the technologies associated with the recent successful development of in vitro fertilization in this species, including estrus hormone monitoring, gamete collection, sperm capacitation, fertilization, and embryo culture, cryopreservation and transfer. 

    Learning Objectives:

    • Describe unique aspects of female canid reproductive biology and how they influence development of ART
    • Identify modifications in dog IVF protocols that led to the successful development of this technique

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