FEB 08, 2017 06:00 AM PST

Producing Petri Puppies: Gamete Collection and In Vitro Fertilization in the Domestic Dog

C.E. CREDITS: RACE
Speakers
  • Postdoctoral Fellow, Smithsonian Conservation Biology Institute
    Biography
      Jennifer Nagashima is a Smithsonian Institution postdoctoral fellow for the Smithsonian Conservation Biology Institute (SCBI) with Nucharin Songsasen. Her current research focuses on developing new "gamete rescue" technologies in canids and felids, include freezing sperm and culturing ovaries in vitro to grow mature eggs.

      Nagashima earned her bachelor's degree from Cornell University in animal science in 2009 and completed her doctorate in zoology and wildlife conservation in 2015. As part of her doctoral dissertation in the Joint Graduate Training Program (JGTP) between Cornell University and SCBI, Nagashima produced the world's first litter of puppies from in vitro fertilization and cultured domestic dog ovarian follicles using cutting-edge, three-dimensional systems, with the goal of applying these technologies to the conservation of wild canids in the future.

    Abstract:

    DATE: February 8, 2017
    TIME: 6:00am PT, 9:00am ET

    Development of assisted reproductive technologies in the dog has lagged behind many other domestic and laboratory animal species, due in part to their unique reproductive biology. Here, we will discuss the challenges with dog ART and the technologies associated with the recent successful development of in vitro fertilization in this species, including estrus hormone monitoring, gamete collection, sperm capacitation, fertilization, and embryo culture, cryopreservation and transfer. 

    Learning Objectives:

    • Describe unique aspects of female canid reproductive biology and how they influence development of ART
    • Identify modifications in dog IVF protocols that led to the successful development of this technique

    Show Resources
    You May Also Like
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    MAY 02, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 02, 2018 08:00 AM PDT
    Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    Loading Comments...
    Show Resources
    Attendees