NOV 10, 2016 07:30 AM PST

Does your protein analysis toolbox need a refresh?

Speakers
  • Senior Marketing Manager; Beckman Coulter
    Biography
      Dr. Jones has a long-standing interest in the development and application of automated protein analysis technologies and workflows for interrogating protein systems in human biology and pharmacology. He has over 20 years of experience in the field of protein and cell biotechnology. While an Assistant Professor in the Ben May Department for Cancer Research at the University of Chicago, Dr. Jones developed micro-western arrays for studying the responses of cancer cells to therapeutics and for understanding chemotherapeutic resistance mechanisms. In addition to being an Assistant Professor, he was a Core Fellow and founding member of the Institute for Genomics and Systems Biology and was founder and director of the University of Chicago Micro-western array Protein Analysis Core Facility.

      He trained at Harvard University as a Kirchstein honorary Postdoctoral Research and Teaching Fellow where he participated in the development of protein microarray approaches for mapping human protein interaction networks in breast cancer.

    Abstract:

    DATE:  November 10, 2016
    TIME:  7:30am PT, 10:30am ET 

    Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each might best be employed in your daily research activities?
     
    High quality proteins are critical for contemporary biomedical research. Biochemists and biophysicists require high quality proteins to probe the molecular mechanisms of proteins in their native state. Proteomics researchers, cell biologists, and pathologists require high-quality antibodies as purification tools and as diagnostic reagents. Formulation scientists need to quickly gain an understanding of the impact that buffer and other components have on the structure and function of protein biologics.
     
    In this webinar, several complementary protein analysis tools will be introduced and discussed including chromatography, UV/VIS spectroscopy, light scattering, electrophoresis, western blotting and analytical ultracentrifugation.  In closing, I will discuss our work in measuring the pairwise interaction matrices of hundreds of ErbB signaling proteins and peptides to illustrate the importance of quality control in obtaining high quality protein interaction data.
     
    Learning Objectives:

    • Learn about approaches for the purification and quality analysis of proteins and antibodies
    • Learn about systematic methods for assessing interaction potentials of proteins 
    • Learn about higher-throughput western blotting methods

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