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NOV 10, 2016 7:30 AM PST

Does your protein analysis toolbox need a refresh?

Speaker

Abstract

DATE:  November 10, 2016
TIME:  7:30am PT, 10:30am ET 

Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each might best be employed in your daily research activities?
 
High quality proteins are critical for contemporary biomedical research. Biochemists and biophysicists require high quality proteins to probe the molecular mechanisms of proteins in their native state. Proteomics researchers, cell biologists, and pathologists require high-quality antibodies as purification tools and as diagnostic reagents. Formulation scientists need to quickly gain an understanding of the impact that buffer and other components have on the structure and function of protein biologics.
 
In this webinar, several complementary protein analysis tools will be introduced and discussed including chromatography, UV/VIS spectroscopy, light scattering, electrophoresis, western blotting and analytical ultracentrifugation.  In closing, I will discuss our work in measuring the pairwise interaction matrices of hundreds of ErbB signaling proteins and peptides to illustrate the importance of quality control in obtaining high quality protein interaction data.
 
Learning Objectives:

  • Learn about approaches for the purification and quality analysis of proteins and antibodies
  • Learn about systematic methods for assessing interaction potentials of proteins 
  • Learn about higher-throughput western blotting methods

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