OCT 08, 2015 8:00 AM PDT

WEBINAR: Proteome Profiling of the Tumor Microenvironment: Role of Human Primary Fibroblasts Derived Exosomes in Oral Cancer Progression

Speakers
  • Post-Doctoral Fellow, Princess Margaret Cancer Center, University Health Network
    Biography
      Simona Principe completed her PhD in Immunopharmacology in Italy (2011) and she is currently conducting postdoctoral research in the lab of Dr. Thomas Kislinger at the Princess Margaret Cancer Center in Toronto, Canada. Her research interest focuses on applying proteomics technologies to investigate cancer biology. In particular, she studies the role of secreted extracellular vesicles as novel intracellular signaling mediators within the tumor microenvironment that support cancer progression and metastasis. Since 2012 she has published 5 papers/reviews in the field of proteomics and exosomes.

    Abstract:
    During the development of oral cancer (OC), cancer-associated fibroblasts (CAFs) create a supporting niche by maintaining a bidirectional crosstalk with cancer cells, mediated by classically secreted factors and nanometer-sized vesicles, called exosomes. Little is known about the molecular alterations in CAFs that determine their unique functional properties and how exosomes contribution to OC progression. To investigate stromal heterogeneity in OCs we isolated and characterized human fibroblasts from resected tumors (CAFs) and adjacent tissue (AFs). We employed shotgun proteomics to identify CAF-associated proteins in total lysate, conditioned media and exosomes. Our comprehensive dataset of 4247 proteins represents a detailed signature of a pro-tumorigenic stroma. Gene ontology enrichment analysis has shown that proteins involved in regulation of cancer cell migration are enriched in the exosomal fraction. To validate our findings we have shown a significant effect of CAF-derived exosomes on OC cells motility, proliferation and the activation of the MAPK pathway. Quantitative proteomics was then applied to highlight differentially expressed proteins in exosomes from CAFs and AFs and generate a short list of proteins specific to the CAF-like state. Significant differences were observed in proteins involved in metabolism, suggesting that metabolic synergy between CAFs and cancer cells could involve exosomal cargo. In summary, integrative proteomics identified a signature of CAF-enriched exosomal proteins involved in pathways mediating tumor-stromal crosstalk. Here, we show that fibroblast-secreted exosomes affect OC cells growth, motility and glucose metabolism. Our data provide a foundation to investigate how the tumor microenvironment affects OC progression.

    Learning Objectives:
    • Learn how quantitative proteomics is applied to highlight differentially expressed proteins in exosomes from cancer-associated fibroblasts (CAFs) and adjacent tissue (AFs)
    • Learn how fibroblast-secreted exosomes affect OC cells growth, motility and glucose metabolism

    Show Resources
    You May Also Like
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    Loading Comments...
    Show Resources