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SEP 17, 2020 9:00 AM PDT

Purification of cells from SARS-CoV-2 convalescent samples and potentially infectious materials with fluorescent-activated cell sorting

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Flow Cytometry Specialist, Miltenyi Biotec
    Biography

      Joe began his flow cytometry career in graduate school by sorting genetically engineered microalgae for use. After completing his doctorate degree and joining Miltenyi Biotec as an Application Scientist, he became proficient in another area of flow cytometry; one focused on clinical assay development, regulatory compliance, and validation. As a Flow Cytometry Specialist, Joe has partnered with a variety of flow experts, ranging from individual researchers in academia all the way to large pharmaceutical companies in order to support and build multifaceted cytometry solutions to fit their diverse laboratory needs. When he is not analyzing results, he spends most of his free time wrangling his one-year old son, picking up the destruction left behind, and  keeping him from falling down the stairs.


    Abstract

    In recent months, we have seen a large part of the global scientific community shift to infectious disease research. In response, researchers are exploring safer and easier methods to handle, isolate, and analyze cells from hazardous materials. Mitigating risk while achieving highly pure, viable cells is essential to understanding the immune system’s role in COVID-19 infection and recovery. When it comes to cell sorting, samples, such as SARS-CoV-2 convalescent samples, virus-infected human cells (e.g., from HIV-positive donors), or bacteria, can escape the fluidic system of a traditional droplet-based cell sorter as aerosols.  Our multi-parametric cell sorting platform offers a solution with its unique closed, single-use, and disposable sorting cartridge. This cartridge provides an increased level of biocontainment and can mitigate the risk when implementing a fluorescent-activated cell sorter in BSL-3 or BSL-2+ level laboratories.

    Learning Objectives:

    1. Scientists utilizing cell sorting to obtain high quality cell populations from biohazardous samples

    2. Mitigating risk while sorting hazardous samples though leveraging a microfluidic-based fluorescent-activated cell sorter

    3. GMP-compliant cell sorting options available for your translational research


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