SEP 26, 2018 6:00 AM PDT

Quantitative Label-Free Imaging and It's Many Applications

Presented at: Cell Biology 2018
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Director of the Bioscience Technology Facility, Head of Imaging Cytometry, Department of Biology, University of York
    Biography
      Peter is Director of the Bioscience Technology Facility at the University of York covering the broad technologies required for a multi-disciplined Department of Biology.

      He has also built up and heads the highly successful Imaging and Cytometry Labs which includes an array of confocal microscopes, flow cytometers, electron microscopes and novel instrumentation. Peter gained his PhD in the Cell Biophysics Laboratory at Essex and has been involved in many aspects of fluorescence imaging and cytometry. Research is currently focused on both technology and method development of novel probes and imaging modalities. His lab provides research support to many academics and commercial organisations and he has ongoing collaborations and consultancy contracts with many leading microscopy and cytometry companies.

      Peter serves on an array of committees for e.g. BBSRC , Vice-President of the RMS and Steering committee for ELMI. Peter is also heavily involved with microscopy and cytometry training through a variety of international courses.
    • Biological Applications Scientist at Phasefocus Ltd.
      Biography
        Rakesh Suman (PhD in Neuroscience, University of Leeds), has been working as an application scientist with Phasefocus for over 4 years. During his time at Phasefocus he has played a crucial role into the evolution of the Livecyte. He has also pioneered and supported the development of numerous label-free assays that are currently available to run on the Livecyte such as, cell proliferation\death; cell motility and migration; angiogenesis; mitotic time and scratch wound healing assays.

      Abstract:

      Quantitative Phase Imaging (QPI) is a rapidly developing area that permits label-free live cell imaging. York has been at the forefront of developing applications through the use of ptychography, a unique form of QPI. This has focussed on longitudinal studies of cell behaviour and looking at the QPI metrics that can be used to identify unique phenotypes and characteristics of single cells and populations. We will look at how we study cells at high confluency levels and look at the unique measurements1 that can be made along, as well as the addition information that can be gained through studying changes in cell motility within mixed cultures2.  We will also look at a variety of new applications still under study here at York along with external collaborators around the world.

      Learning Objectives: 

      1. An understanding of label free microscopy, quantitative phase  imaging and ptychography.
      2. The many potential applications for time lapse studies using QPI.
      3. How label free imaging fits into a multi core lab.


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