APR 20, 2021 7:00 AM PDT

Rapid biotherapeutic charge variant analysis by CZE-UV and CZE-MS

Sponsored by: SCIEX
Speakers
  • Accurate Mass Workflow Specialist
    Biography
      Christian Baumann studied Chemical Engineering at Fresenius in Idstein, Germany, with a focus on instrumental analytics such as LC-MS, GC-MS and NMR. Christian worked for ten years at Aventis/Proteome Sciences as Head of the Mass Spectrometry Group, contributing in development, QC analysis and applications of isobaric mass tags for peptide quantification in the proteomics field. He joined SCIEX in 2011 as an Application Support Specialist, focusing on proteomics and biologics, before moving into the role of Accurate Mass Workflow Specialist within the Sales organization.
    • Field Applications Specialist
      Biography
        Whitney Smith received her Ph.D. in analytical chemistry in 2011 from the University of Oklahoma with a focus on GC- and LC-MS. After completing a post-doc using LC-MS for metabolomics, she moved to Europe to start her career with SCIEX. She began as a CE service engineer and transferred to CE application support in 2016. In her current role, she supports applications with optical and mass spectrometric detection.

      Abstract
      Date:  April 20, 2021
      Time: 7:00am (PDT),  10:00am (EST)
       
      There is a vital need to characterize Biopharmaceuticals in early phase screening to ensure the most suitable candidates are accelerated through the development funnel. Charge variants and amino acid sequence variants are critical quality attributes (CQAs) which need to be characterized and controlled quickly during screening and process development.
       
      Rapid charge variant analysis is performed by gold standard CZE and cIEF capillary electrophoresis methods with optical detection. However, identification of unknown charge variant peaks remains a challenge without complex workflows involving fraction collection. The work presented here demonstrates that CE-MS profiles near identical to those from CZE and cIEF allows identification of unknown charge variant peaks in under 10 minutes.
       
      What you will learn:
      • Understand why capillary electrophoresis is an ideal technique to quantify and identify protein charge variants by optical and mass spectrometry detection.
      • Learn how to obtain robust quantitative data by combining fast CE separation and rapid MS acquisition speed.
       
      Webinars will be available for unlimited on-demand viewing after live event.

      Show Resources
      You May Also Like
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      NOV 18, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 18, 2020 8:00 AM PST
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      OCT 29, 2020 6:00 AM PDT
      C.E. CREDITS
      OCT 29, 2020 6:00 AM PDT
      Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
      Loading Comments...
      Show Resources
      Attendees
      • See more