Rapid hyper-plex staining and simultaneous imaging for immunophenotyping of tissue sections.

 

The simultaneous detection of biomarkers on tissue samples plays a fundamental role in the study of the tumor microenvironment. Multiplexed immunofluorescence tools have demonstrated to be key enablers in these endeavors. Available techniques to perform high-plex staining require intensive manual handling, are highly time-consuming, or require special labeling of primary antibodies.

 

Here we present a highly automated, extremely rapid hyper-plex approach to stain and image tissue samples using label-free primary antibodies in combination with off-the-shelf fluorescently labeled secondary antibodies.