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Real-time Live-cell Analysis for Immunologists

Speaker

Abstract

Deriving deeper biological insight & improving productivity in immune-cell biology

The most common in vitro methods for immunologists to analyze cells of the immune system are flow cytometry, PCR and various forms of ELISA. Together, these enable quantification of different cell populations at the molecular and functional level (‘immuno-phenotyping’) and measurement of immune responses, for example, cytokine release. While these platforms are extremely powerful, they provide little insight into morphology and the spatial interactions of cells. Moreover, as ‘end-point’ measures, they are not well suited to report on the dynamic changes in biology over time.

In this webinar, Dr. Del Trezise discusses:

  • Real-time live-cell analysis
  • The use of automated time-lapse imaging for the kinetic quantification of and functional analysis of immune cells
  • A number of case studies including T cell proliferation and clustering, phagocytosis, chemotaxis, cytolysis and viral infection assays

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Real-time Live-cell Analysis for Immunologists



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