MENU

Real-Time Monitoring of Striatal GPCR Mediated cAMP Signaling Using Genetically Encoded Fluorescent Sensors

Presented at: Neuroscience 2020
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Research fellow, National Institutes of Health (NIH)
    Biography
      Dr. Augustin received her PhD for her work in Neuroscience from the University of Chicago, IL, USA in 2013, where her work focused on elucidating the role of cyclic AMP in corticostriatal synaptic plasticity. Following her graduate studies, Dr. Augustin joined the Laboratory for Integrative Neuroscience at the National Institute on Alcohol Abuse and Alcoholism as an Intramural Research Training Award postdoctoral fellow (NIAAA-IRTA). There, she has continued her research examining the cellular mechanisms of corticostriatal synaptic plasticity focusing on the involvement of different striatal D2 dopamine receptor populations in the induction of synaptic plasticity. Also, she has worked on projects investigating the functional role of endocannabinoid transmissions in synaptic plasticity and behavior. Dr. Augustin was the recipient of an NIH BRAIN Initiative advanced postdoctoral career transition award (K99/R00). For her BRAIN Initiative project, she will examine the cellular and subcellular signaling pathways (e.g. cyclic AMP and PKA) during the induction corticostriatal plasticity and during striatal-based behaviors using genetically encoded sensors and optical techniques.

    Abstract

    Striatal neuromodulation through G-protein-coupled receptors (GPCRs) regulates complex voluntary motor actions, involving decision-making, learning, and action selection. The dorsal striatum integrates information from neuromodulatory inputs from the thalamus, midbrain, and various parts of cortex, as well as intrinsic neuromodulators within in its microcircuity. The integration of these signals initiates the communication of information through the basal ganglia to control action learning and performance. Many of these inputs form synapses onto indirect and direct-pathway projection medium spiny neurons (MSN). Both subpopulation of MSNs contains GPCRs that can oppositely regulate the cAMP-PKA signaling pathway. This signaling pathway is critical in the modulation of synaptic transmission, long-term synaptic plasticity and learning and memory. In the striatum, the dynamic interplay between dopamine and cAMP-PKA signaling is important for the induction of long-term depression. However, the spatial and temporal dynamics of these intracellular effector molecules in real-time during the induction of synaptic plasticity and behavior remain unclear. Using newly optimized intensity- and FRET-based genetically encoded cAMP biosensors together with optical techniques, we can now measure activity-induced changes in cAMP accumulation in real-time in live brain slices and in freely moving animals. Simultaneous recordings of activity-induced cAMP transients detected by cAMP Difference Detector in situ (cADDis) intensity-based sensor and dopamine transients were performed using slice photometry and fast-scan cyclic voltammetry, respectively. Single pulse electrical stimulation generates increased cAMP in D1 dopamine receptor-expressing MSNs and decreased cAMP in D2 receptor expressing MSNs. These signaling changes coincide with increased extracellular dopamine. We are currently examining the kinetics of the dopamine and cAMP signals. Both transients can be altered by changes in stimulation duration and frequency, and are blocked by tetrodotoxin, indicating that they are driven by neuronal activation. Our findings indicate the feasibility of using optical biosensors combined with traditional methods to investigate precise temporal dopamine-cAMP dynamics in real-time in the striatum.

    Learning Objectives:

    1. Learn about newly developed and optimized genetically encoded fluorescent sensors to measure second messenger effector molecules

    2. Explain optical imaging tools to measure these sensors in vitro (live brain slices) and in vivo during freely moving behaviors


    Show Resources
    You May Also Like
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    SEP 02, 2020 7:00 AM PDT
    C.E. CREDITS
    SEP 02, 2020 7:00 AM PDT
    DATE: September 2, 2020 TIME: 03:00pm PDT, 6:00pm EDT Spatial omics is an expanding collection of methods to examine biological molecules in their geographical context. By retaining the prec...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    Loading Comments...
    Show Resources
    Attendees
    • See more