JUL 28, 2020 10:00 AM PDT

Rebuilding evolutionary success: Using Inscripta's Onyx Digital Genome Engineering Platform to ease and enhance Adaptive Laboratory Evolution experiments

Sponsored by: Inscripta
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist II, Applications Development, Inscripta
    Biography

      Dr. Tyson Shepherd has a background in biochemistry and synthetic biology, publishing on protein engineering, large DNA construct design, cell-free synthetic biology, phage biology, and nucleic acid nanotechnology. Dr. Shepherd works in a collaborative team within Inscripta and with external partners to design and execute projects demonstrating the many applications of the Onyx Digital Genome Engineering Platform. Before joining Inscripta, Dr. Shepherd was a research scientist at MIT. Dr. Shepherd earned his graduate degree at the University of Iowa and did postdoctoral research at MIT and Uppsala University.


    Abstract

    DATE:  July 28, 2020

    TIME:   10:00am PDT, 1:00pm EDT

     

    Adaptive laboratory evolution (ALE) of microbial strains is a powerful approach in biotechnology to generate chemical tolerance and gain novel biochemical insights. However, these experiments are often lengthy in their execution (weeks to decades), require strain rebuilds for validation, and can suffer from evolutionary traps. Massively parallel microbial strain engineering using Inscripta’s Onyx™ benchtop platform enabled high throughput design and build of a barcoded library of 372 individually reconstructed, previously reported ALE strain variants, 492 gene deletions, and 84 mobile element-like insertions. This library was tested against inhibitory concentrations of NaCl, coumaric acid, and isobutyric acid for rapid selection of beneficial mutations. The application of this technology to ALE variant reconstruction has allowed for an unprecedented opportunity to query a diverse population of mutations for their role in growth responses to gain new biochemical insights. The high-throughput capabilities of this strain construction platform have enabled a new paradigm in ALE experiments and offer the opportunity to do the same across countless additional applications in both basic and applied biological sciences.

     

    Learning Objectives:

    • Introduction to Inscripta’s Onyx™ Digital Genome Engineering Platform

    • Example application of genome-wide strain engineering in E. coli

    • Reported adaptive laboratory evolution (ALE) variants were built in high throughput and barcode tracked to enable genotyping tolerance mutations

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing


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