JUL 28, 2020 10:00 AM PDT

Rebuilding evolutionary success: Using Inscripta's Onyx Digital Genome Engineering Platform to ease and enhance Adaptive Laboratory Evolution experiments

Sponsored by: Inscripta
Speaker

Abstract

DATE:  July 28, 2020

TIME:   10:00am PDT, 1:00pm EDT

 

Adaptive laboratory evolution (ALE) of microbial strains is a powerful approach in biotechnology to generate chemical tolerance and gain novel biochemical insights. However, these experiments are often lengthy in their execution (weeks to decades), require strain rebuilds for validation, and can suffer from evolutionary traps. Massively parallel microbial strain engineering using Inscripta’s Onyx™ benchtop platform enabled high throughput design and build of a barcoded library of 372 individually reconstructed, previously reported ALE strain variants, 492 gene deletions, and 84 mobile element-like insertions. This library was tested against inhibitory concentrations of NaCl, coumaric acid, and isobutyric acid for rapid selection of beneficial mutations. The application of this technology to ALE variant reconstruction has allowed for an unprecedented opportunity to query a diverse population of mutations for their role in growth responses to gain new biochemical insights. The high-throughput capabilities of this strain construction platform have enabled a new paradigm in ALE experiments and offer the opportunity to do the same across countless additional applications in both basic and applied biological sciences.

 

Learning Objectives:

  • Introduction to Inscripta’s Onyx™ Digital Genome Engineering Platform

  • Example application of genome-wide strain engineering in E. coli

  • Reported adaptive laboratory evolution (ALE) variants were built in high throughput and barcode tracked to enable genotyping tolerance mutations

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
MAR 30, 2022 6:00 AM PDT
MAR 30, 2022 6:00 AM PDT
Targeted NGS has been instrumental in helping the healthcare community deliver on the promise of precision medicine. The Ion Torrent Genexus Integrated Sequencer has enabled targeted genomic...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
AUG 17, 2022 10:00 AM PDT
AUG 17, 2022 10:00 AM PDT
DATE: October 22, 2022 TIME: 8:00am PDT, 11:00am EDT Detection and/or amplification of target DNA sequences by polymerase chain reaction (PCR) is a prevalent method in molecular biology. PCR...
Loading Comments...
Show Resources