MENU

Reprogrammed stem cells to study psychedelic substances

Speaker
  • Head of Research Professor of Biomedical Sciences, D'Or Institute for Research and Education (IDOR) & Institute of Biomedical Sciences Federal University of Rio de Janeiro
    Biography
      Stevens Rehen received his Bachelor's degree, Master's degree, and Ph.D. at the Federal University of Rio de Janerio in Brazil. He later completed postdoctoral training at the University of California San Diego and The Scripps Research Institute. Over the past five years, Stevens has published over 76 peer-reviewed publications. Currently, Stevens is a Full Professor at the Federal University of Rio de Janerio. Additionally, he is the Head of Research at D'Or Institute for Research and Education (IDOR) and Regional Committee Member of the Pew Latin American Program in the Biomedical Sciences

    Abstract

    For more than four decades, restrictions on research with psychoactive drugs have slowed progress in understanding how such substances impact brain metabolism. Besides the historical restrictions, the impacts of drug exposure in human neural cells have been compromised by limitations of adequate models. I will present the effects of the β-carboline alkaloid harmine, component of the psychoactive plant tea known as “Ayahuasca”, and 5-MeO-DMT (5-methoxy-N,N-dimethyltryptamine), found in the Sonora Desert toad, in cultures of human neural cells and brain organoids derived from induced pluripotent stem cells. Harmine increased the pool of proliferating cells, with DYRK1A (dual specificity tyrosine-phosphorylation-regulated kinase) as a target, which suggests a biological activity possibly associated with the antidepressant effects of Ayahuasca in patients with depressive disorder. Analyzing global protein expression of brain organoids exposed to 5-MeO-DMT, we found proteins broadly distributed on functional activities such as cellular protrusion formation, microtubule dynamics and cytoskeletal reorganization, which are correlated to novel dendritic spine formation. These models offer an exciting new range of opportunities to investigate the impact of psychedelics on human neural cells.           


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    JUN 09, 2020 3:00 PM CEST
    C.E. CREDITS
    JUN 09, 2020 3:00 PM CEST
    DATE: June 9, 2020 TIME: 6am PT, 9am ET, 3pm CEST The importance of disposable plastic consumables and their overall impact on the experimental workflow of qPCR has been taken into considera...
    Loading Comments...
    Show Resources
    Attendees
    • See more