MAR 26, 2019 2:00 PM PDT

The Role of Endosomes in Prostate Cancer

Speaker

Abstract
DATE:  March 26, 2019
TIME:  2:00pm PDT, 5:00pm EDT
 
Prostate cancer is the second most common form of cancer in males, affecting one in eight men by the time they reach the age of 75. The disease continues to be a major cause of morbidity and mortality in men, but a method for accurate prognosis in these patients is yet to be developed necessitating a search for new molecular markers and continued investigation of prostate cancer cell biology.
Cell lines have been used in the search for biomarkers that are suitable for prostate cancer diagnosis. Many studies have only involved single cell lines, partially characterised cell lines or were performed without non-malignant controls, potentially undermining effective biomarker discovery.

By utilising a larger panel of prostate cell lines, we discovered that cell lines transfected with HPV-18 display an aberrant pattern of protein and gene expression compared to SV40 or non-immortalised cancer cells. Through this discovery process we found that the critical process of endosomal biogenesis might be altered in prostate cancer. Microarray analysis of clinical cohorts confirmed these changes and were further delineated in fresh-frozen prostate cancer tissue by qRT-PCR.

The discrimination of prostate cancer cell lines can be further achieved using a various spectroscopic methods; utilising quantitative mass spectrometry (LC-ESI-MS/MS), non-invasive FTIR analysis and fluorescent detection of lipids in live cells by imaging with confocal microscopy we identified specific phospholipids and cholesteryl ester species that could distinguish between aggressive and non-aggressive cellular phenotypes and that could act as potential biomarkers for prostate cancer. It is possible to discriminate between cancer and non-malignant cells using a luminescent metal complex ReZolve-L1 that interacts with polar lipids. These technologies can be employed to find new candidates that have significant biomarker potential.
 
Learning Objectives:
  • Understand the roles of endosomes and lysosomes in prostate cancer
  • Explore how endosomes can influence cancer cell biology and development

 

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
MAR 26, 2019 2:00 PM PDT

The Role of Endosomes in Prostate Cancer



Show Resources
Loading Comments...
Show Resources