NOV 24, 2015 06:00 AM PST

Say goodbye to Gels - Real world use of LabChip micro-fluidic technology for nucleic acid and protein analysis

SPONSORED BY: PerkinElmer, Inc., PerkinElmer, Inc.
6 26 7442

  • Staff Scientist, Laura Bassi Center for Optimized Structural Studies
      Dr. Kostan received his PhD in Molecular biology from the University of Vienna working on protein-protein interactions of cytoskeletal associated proteins in the group of Gerhard Wiche (MFPL, Vienna). Eight years ago he joined the group of Kristina Djinovic-Carugo (MFPL, Vienna) with the main focus on protein crystallography, biophysical and biochemical characterization of proteins and protein complexes, protein purification and structural biology of F-actin based cytoskeleton. During the last three years he worked as a staff scientist in the Laura Bassi Center for Optimized Structural Studies (COSS), a collaborative research project coordinated by Kristina Djinovic-Carugo. The main interest of COSS is the research of innovative methods for production of sufficient quantities of high-quality and functionally active proteins for structural and functional analysis, nowadays focusing mainly on the production, and structure determination of protein complexes involving proteins of the Z-disk interactome.
    • Medical Scientist, Pathwest Clinical Immunology at Fiona Stanley Hospital
        My name is Sandra Doran and I am a Medical Scientist for Pathwest Clinical Immunology at Fiona Stanley Hospital in Perth, Western Australia. HLA Typing is a large component of the work performed by Clinical Immunology primarily for solid organ and bone marrow transplantation. The majority of my experience in the department has involved working with the molecular based HLA typing assays. Originally this involved Sanger Sequence based typing methods and more recently I have been heavily involved in the implementation of Next Generation Sequencing (NGS) using the Life Technologies Ion Torrent platform.

        Gel electrophoresis has been used in our department routinely for the detection of HLA PCR amplicons prior to downstream sequencing methods. The method is limiting as it does not provide quantitative analysis of samples and cannot distinguish Polymerase Chain Reaction (PCR) amplicons where the size difference is less that 50 base pairs. The LabChipGX was acquired as the switch to Next Generation Sequencing protocols required sizing and quantitation of samples at various points in the process. An extensive validation process was carried out to ensure that the instrument was appropriate for our NGS workflow and could replace gel electrophoresis for other routine assays offering smoother work flow and the chance to remove the use of ethidium bromide. The LabChipGX assays validated and implemented into routine processes include the HT DNA 5K assay, for quantitation of PCR amplicons, and the High Sensitivity assay, for sizing and quantitation of NGS libraries. I will be presenting the data obtained in the validation process along with information about how the LabChipGX fits into our routine workflow and has now become an essential instrument in our daily operations.
      • Field Application Scientist with Perkin Elmer
          Veronika Delcheva is a Field Application Scientist with Perkin Elmer since December 2012. Together with her team, she is responsible for pre- and post-sales projects covering the Microfluidics (LabChip)Product Portfolio in Europe. She has a Dipl.-Ing. (equil. M.Sc) Biotechnology Degree from the University of Applied Sciences in Darmstadt, Germany. Prior to joining PerkinElmer, Veronika was a scientific employee at the molecular-genetic laboratory of the psychiatric, psychosomatic and psychotherapeutic clinic of the infantile and adolescent age at the University Clinic in Frankfurt/Main, where she participated in projects related to the research of the risk factor for autistic spectrum disorders.

        DATE: November 24, 2015
        TIME: 9am EST, 2pm GMT

        For many years now, gels have been a fundamental research tool in academic research for the separation and analysis of nucleic acids and proteins. Recently, new replacement technologies have emerged that have become industry standard in biotech and pharma and address some of the limitations of gels.  Generally gels are not simple to setup, take hours to run and require post-run steps to document and store the resulting data. Often, the data storage medium is an acetate or photograph, which leads to archiving, retrieval and security issues. Safety is an issue, particularly if Ethidium Bromide is used. Finally, as experienced gel users leave the lab,  knowledge is lost leading to variability in performance and resolution and of course, the ubiquitous “smile”.
        LabChip technology runs samples in under one minute, automatically archives the data electronically, produces high resolution, highly reproducible data and is easy to setup. In this web-seminar, users discuss their real-world experience of using  LabChip technology to replace gels and how the methods compared with each other.  Sandra Doran discusses how LabChip technology changed the workflow at the PathWest Laboratory, away from agarose gel based analysis of nucleic acids. Dr Julius Kostan of the structural laboratory at the University of Vienna, discusses how LabChip allowed them to move away from SDS-PAGE gels for protein analysis and finally Veronika Delcheva, Application Specialist in the Microfluidics application support group of PerkinElmer, will explain how the technology works and the practicalities of setting up a run.

        The per-sample cost of running LabChip technology is comparable and frequently less expensive than pre-cast gels so there’s now no reason why academic researchers shouldn’t be benefiting from this technology revolution. This webinar will allow you to hear and put your questions to users who have made the change and judge for yourself if it’s time to start thinking about saying goodbye to gels

        Show Resources
        Loading Comments...