MAY 10, 2017 02:00 PM PDT

Selecting the right resin to achieve optimal results, from high throughput batch to process scale protein purification

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  • Scientist III, Protein and Cell Analysis, Life Sciences Solutions Group, Thermo Fisher Scientific
      Aaron McBride is an R&D Scientist at Thermo Fisher Scientific, where he has played an instrumental role in the development, commercialization and support of multiple chromatography resins and recombinant proteins introduced over the past seven years. Aaron has experience in protein expression, purification and characterization, as well as, process development and validation. HIs development work included products for protein purification, molecular biology and in vitro translation. His formal education includes an MS in Cell and Molecular Biology from Michigan State University.
    • Scientist III, Protein and Cell Analysis, Life Sciences Solutions Group, Thermo Fisher Scientific
        Scott Meier is a Research Scientist at Thermo Fisher Scientific, focusing primarily on the development of novel tools for protein isolation and enrichment. He has a strong background in protein expression and purification for structural and functional studies. His most recent project involved developing magnetic agarose affinity beads for high-throughput small scale purification of recombinant proteins and antibodies. Over the past six years, Scott has developed products related to bacterial lysis, membrane protein extraction, high flow affinity chromatography resins, and glycoprotein immobilization chemistry. Scott has a B.S. in Bioengineering from University of Illinois Urbana-Champaign and a M.S. in Laboratory Investigation from Vanderbilt University School of Medicine.


      The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards that pursuit, proper chromatography resin selection is essential to achieving a high efficiency purification strategy, which includes optimal protein separation and minimal processing time. In this presentation, we discuss a full suite of resins that can be utilized for microgram to kilogram scale purifications. Whether screening at a high throughput batch scale to select the right target, to increasing production of that target protein from batch to process scale, we will be highlighting the technological advantages and cost-savings of our full portfolio of base beads, from convenient small scale processing using magnetic agarose to simplified scale up using the uniquely designed Thermo Scientific POROS™ resin bead, that allows for enhanced resolution and faster processing.

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