DEC 10, 2020 8:00 AM PST

Simple and Fast Charge Variants Analysis of mAbs by CZE

Sponsored by: SCIEX
Speaker

Abstract
Date:  December 10, 2020
Time: 8:00am (PST),  11:00am (EST)
 
As you face increasingly shortened timelines to bring new drugs to market, you find yourself strained to finalize analytical methods faster than ever before. Of all methods, charge variant analysis can be the most time consuming, and one of the most important as it is up to you to identify and analyze these attributes including stability, efficacy and potency. The two traditional techniques for monitoring and analyzing these variants are HPLC- based ion exchange chromatography (IEX) and capillary isoelectric focusing (cIEF/iCIEF). However, both of these techniques have drawbacks: it can take considerable time to develop a molecule specific method and these methods generally analyze a molecule in its denatured state. Fortunately, an alternative technique known as capillary zone electrophoresis (CZE) can provide a much higher analytical throughput and little to no method optimization and sample preparation. It is quickly becoming a readily used method for development and quality control release assays. Not only is CZE fast and reproducible, it is also a high resolution native state analysis.
 
In this webinar, Dr. He will review a new PTM monitoring method utilizing capillary zone electrophoresis (CZE) on two therapeutic grade antibodies in a matter of minutes.
 
Learning Objectives
  • Fundamentals of capillary zone electrophoresis (CZE) for charge variant analysis
  • Advantages of capillary zone electrophoresis (CZE) over alternative charge heterogeneity techniques
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

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