OCT 11, 2017 1:30 PM PDT

Simultaneous multi-analyte profiling of rare antigen specific T cells with 3D Flow™ Analysis

Sponsored by: NanoString Technologies
Speakers
  • Professor, Arizona State University
    Biography
      Dr. Karen Anderson M.D., Ph.D., is a Professor at Arizona State University. She received her M.D. and Ph.D. in Microbiology and Immunology from Duke University. Dr. Anderson is a Registered Physician and a member of the American Association for Cancer Research as well as American Society of Clinical Oncology. Dr. Anderson's laboratory is focused on understanding how the immune response can be used to detect and alter cancer development. The development of effective cancer immunotherapy requires identification of target antigens, and also an understanding of the mechanisms of immune regulation that limit effective immunotherapy. One challenge in tumor immunology is how to measure immune responses across the proteome. Antibodies to tumor antigens can be detected in the blood of cancer patients, and can be used as biomarkers for early cancer detection.
    • Principal Product Application Scientist, NanoString
      Biography
        DOUG HINERFELD, PH.D. is the Principal Product Application Scientist for NanoString 3D Biology™ Technology. In this role at NanoString, he is responsible for the development of novel applications for the 3D Biology portfolio. He received a B.A. in Psychology from The University of Colorado, Boulder and a Ph.D. in Genetics and Molecular Biology from Emory University.

      Abstract

      Recent advances in flow and mass cytometry have greatly expanded the number of immune cell parameters that can be interrogated, resulting in an improved understanding of the immune system heterogeneity. However, despite these advances, the immense variety of immune cell phenotype/function requires increasingly sophisticated analytical approaches to analysis. 

      To help address the need for new analytical techniques of rare flow sorted samples, we developed 3D Flow™ Analysis, a streamlined workflow that seamlessly integrates standard immune cell flow sorting with downstream NanoString nCounter® analysis. This new workflow enables the highly multiplexed digital detection of 770 RNA and 30 proteins from a single biological specimen. 

      In this webinar, we feature Dr. Karen Anderson who utilized 3D Flow Analysis to deeply profile antigen specific T cells. Hear about the exciting biology uncovered from these rare cells utilizing this novel technique.
      FOR REASEARCH USE ONLY Not for use in diagnostic procedures 
       


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