OCT 11, 2017 1:30 PM PDT

Simultaneous multi-analyte profiling of rare antigen specific T cells with 3D Flow™ Analysis

Sponsored by: NanoString Technologies
Speakers
  • Professor, Arizona State University
    Biography
      Dr. Karen Anderson M.D., Ph.D., is a Professor at Arizona State University. She received her M.D. and Ph.D. in Microbiology and Immunology from Duke University. Dr. Anderson is a Registered Physician and a member of the American Association for Cancer Research as well as American Society of Clinical Oncology. Dr. Anderson's laboratory is focused on understanding how the immune response can be used to detect and alter cancer development. The development of effective cancer immunotherapy requires identification of target antigens, and also an understanding of the mechanisms of immune regulation that limit effective immunotherapy. One challenge in tumor immunology is how to measure immune responses across the proteome. Antibodies to tumor antigens can be detected in the blood of cancer patients, and can be used as biomarkers for early cancer detection.
    • Principal Product Application Scientist, NanoString
      Biography
        DOUG HINERFELD, PH.D. is the Principal Product Application Scientist for NanoString 3D Biology™ Technology. In this role at NanoString, he is responsible for the development of novel applications for the 3D Biology portfolio. He received a B.A. in Psychology from The University of Colorado, Boulder and a Ph.D. in Genetics and Molecular Biology from Emory University.

      Abstract

      Recent advances in flow and mass cytometry have greatly expanded the number of immune cell parameters that can be interrogated, resulting in an improved understanding of the immune system heterogeneity. However, despite these advances, the immense variety of immune cell phenotype/function requires increasingly sophisticated analytical approaches to analysis. 

      To help address the need for new analytical techniques of rare flow sorted samples, we developed 3D Flow™ Analysis, a streamlined workflow that seamlessly integrates standard immune cell flow sorting with downstream NanoString nCounter® analysis. This new workflow enables the highly multiplexed digital detection of 770 RNA and 30 proteins from a single biological specimen. 

      In this webinar, we feature Dr. Karen Anderson who utilized 3D Flow Analysis to deeply profile antigen specific T cells. Hear about the exciting biology uncovered from these rare cells utilizing this novel technique.
      FOR REASEARCH USE ONLY Not for use in diagnostic procedures 
       


      Show Resources
      You May Also Like
      JAN 23, 2020 9:00 AM PST
      C.E. CREDITS
      JAN 23, 2020 9:00 AM PST
      DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
      APR 07, 2020 8:00 AM PDT
      C.E. CREDITS
      APR 07, 2020 8:00 AM PDT
      DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
      MAY 08, 2020 10:00 AM PDT
      C.E. CREDITS
      MAY 08, 2020 10:00 AM PDT
      DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
      FEB 26, 2020 9:00 AM PST
      C.E. CREDITS
      FEB 26, 2020 9:00 AM PST
      DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
      FEB 25, 2020 9:00 AM PST
      C.E. CREDITS
      FEB 25, 2020 9:00 AM PST
      Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
      MAR 03, 2020 9:00 AM JST
      C.E. CREDITS
      MAR 03, 2020 9:00 AM JST
      DATE: March 3, 2020 TIME: 9:00am JST A major limitation in the ex vivo expansion of harvested human hematopoietic stem-progenitor cells (HSPCs) is the rapid differentiation of HSPCs at the e...
      Loading Comments...
      Show Resources
      Attendees
      • See more