DEC 17, 2019 8:00 AM PST

Single-Cell Phosphoprotein Analysis with CyTOF: From Training to Immuno-Oncology Clinical Research

Sponsored by: Fluidigm
  • Assistant Professor in the Department of Cell & Developmental Biology Scientific Director of the Cancer & Immunology Core and the Mass Cytometry Center of Excellence
      Dr. Irish is Assistant Professor of Cell & Developmental Biology at Vanderbilt University. His lab studies how signaling controls cell identity in healthy human tissues and in diseases, including cancer and immune disorders. His approach emphasizes combined use of bench and computational single-cell techniques. Dr. Irish trained at Stanford University with Dr. Garry Nolan, MD, PhD, and Dr. Ronald Levy, MD, for his PhD and postdoc, respectively. His PhD thesis work created a new single-cell phospho-specific flow cytometry approach and applied it to stratify leukemia patient clinical risks. He also developed the quantitative framework for phospho-flow and wrote the first lines of code for the Cytobank cloud computing platform. As a postdoc, Dr. Irish adapted single-cell tools for lymphoma precision medicine and revealed a new negative prognostic lymphoma cell type defined by abnormal B cell signaling mechanisms. In his independent lab at Vanderbilt, Dr. Irish has focused on adapting the immunologist's toolkit and single-cell snapshot proteomics for human solid tissues and tumors, including melanoma, lung cancer and brain cancer. In the last five years, the lab has authored >40 peer-reviewed manuscripts dissecting cancer and immune cell signaling interactions and created machine learning tools to quantify cell identity.
    • Managing Director of the Cancer & Immunology Core and the Mass Cytometry Center of Excellence
        Ms. Roe is Program Manager in the Irish lab and Managing Director of the Mass Cytometry Center of Excellence and Cancer & Immunology Core at Vanderbilt University. Ms. Roe earned a BS in genetics, cell biology and development from the University of Minnesota Twin Cities and expects to complete an MS in the Master of Laboratory Investigation program at Vanderbilt University in the spring of 2020. Ms. Roe has expertise in phospho-specific flow cytometry, human and mouse tissue preparation and mass cytometry (CyTOF) panel and experimental design. She uses these skills to assist lab members and core customers in a wide variety of projects. Some of her favorite parts of her role in the Mass Cytometry Center of Excellence include opportunities to test new CyTOF reagents and streamline existing workflows. She is currently authoring a new protocol for phospho-specific mass cytometry.


      DATE: December 17, 2019

      TIME: 8:00am PST

      Mass cytometry instruments simultaneously measure >50 cellular features, with a typical experiment including millions of cells. Dysregulated phosphoprotein signaling networks are a primary cause of human diseases, including cancer and autoimmunity. This webinar will be a two-part series from investigators at Vanderbilt University who are using single-cell mass cytometry (CyTOF® technology) to map signaling networks in primary human tissues. Dr. Jonathan Irish and Ms. Caroline Roe direct the Vanderbilt Mass Cytometry Center of Excellence, and they use mass cytometry to map signaling networks in primary human tissues. They will give a pair of talks that focus on topics ranging from graduate student training experiments to standardized assays on clinical research studies.

      Dr. Irish will share data from a phosphoprotein mass cytometry study of human brain tumors. Dr. Irish’s talk will also highlight Risk Assessment Population IDentification (RAPID), an unsupervised machine learning workflow their group developed to identify tumor cell subsets that independently and continuously stratified patient outcomes and harbored opposing signaling axes. Once revealed by high-dimensional cytometry, prognostic glioblastoma signaling profiles were apparent in tissue microarray cores using traditional pathological approaches.

      Ms. Roe will then detail best practices in implementing phosphoprotein analysis using mass cytometry, for those looking to expand or refine their clinical research program. Both speakers will highlight examples where mass cytometry can phenotype cells while reliably measuring multiple phosphoprotein in rare subsets of cells. 


      For Research Use Only. Not for use in diagnostic procedures.



      Webinars will be available for unlimited on-demand viewing after live event. 

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      DEC 17, 2019 8:00 AM PST

      Single-Cell Phosphoprotein Analysis with CyTOF: From Training to Immuno-Oncology Clinical Research

      Sponsored by: Fluidigm



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