DATE: December 17, 2019
TIME: 8:00am PST
Mass cytometry instruments simultaneously measure >50 cellular features, with a typical experiment including millions of cells. Dysregulated phosphoprotein signaling networks are a primary cause of human diseases, including cancer and autoimmunity. This webinar will be a two-part series from investigators at Vanderbilt University who are using single-cell mass cytometry (CyTOF® technology) to map signaling networks in primary human tissues. Dr. Jonathan Irish and Ms. Caroline Roe direct the Vanderbilt Mass Cytometry Center of Excellence, and they use mass cytometry to map signaling networks in primary human tissues. They will give a pair of talks that focus on topics ranging from graduate student training experiments to standardized assays on clinical research studies.
Dr. Irish will share data from a phosphoprotein mass cytometry study of human brain tumors. Dr. Irish’s talk will also highlight Risk Assessment Population IDentification (RAPID), an unsupervised machine learning workflow their group developed to identify tumor cell subsets that independently and continuously stratified patient outcomes and harbored opposing signaling axes. Once revealed by high-dimensional cytometry, prognostic glioblastoma signaling profiles were apparent in tissue microarray cores using traditional pathological approaches.
Ms. Roe will then detail best practices in implementing phosphoprotein analysis using mass cytometry, for those looking to expand or refine their clinical research program. Both speakers will highlight examples where mass cytometry can phenotype cells while reliably measuring multiple phosphoprotein in rare subsets of cells.
For Research Use Only. Not for use in diagnostic procedures.
Webinars will be available for unlimited on-demand viewing after live event.