JAN 25, 2023 10:00 AM PST

Single-Molecule Proteomics using Protein Identification by Short-epitope Mapping

Sponsored by: Nautilus Biotechnology
Speaker

Abstract
Date:  January 25, 2023
Time: 10:00am (PST),  1:00pm (EST), 7:00pm (CET)
 
The field of proteomics is poised for a single-molecule revolution – enabling more comprehensive analysis of the proteins in a sample with increased sensitivity, reproducibility, and accessibility. Here we demonstrate Protein Identification by Short-epitope Mapping (PrISM). PrISM is a single-molecule analysis method where intact proteins are immobilized, iteratively probed with multi-affinity probes, and machine learning is applied to convert binding patterns to protein identification and quantification. PrISM uses non-traditional affinity reagents with high affinity and low specificity that bind to short epitopes in multiple proteins. Simulations using these multi-affinity probes show that the accumulated information from multiple rounds of detection of short (2-4 amino acid) epitopes enables identification of more than 95% of the human proteome with just 300 probes. Model proteins were conjugated to DNA nanoparticles and deposited on a high-density patterned flow cell at optically resolvable locations. We acquired PrISM data using >30 multi-affinity probes targeting trimer or tetramer sequences. We demonstrate identification of individual protein molecules through iterative probing with our multi-affinity probes. By combining single-molecule analysis, intact (non-digested) proteins, and iterative probing, PrISM provides a new tool for the quantitation of proteins enabling new exploration of the proteome.
 
Learning Objectives
  • Understand the benefits of single-molecule protein analysis
  • Learn about the PrISM approach to measuring the proteome at scale
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
SEP 22, 2022 9:00 AM PDT
SEP 22, 2022 9:00 AM PDT
Date: September 22, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Optimizing platforms for surgical specimen collection and deep human phenotyping was used to enhance protein biomarke...
OCT 13, 2022 9:00 AM +08
OCT 13, 2022 9:00 AM +08
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
Loading Comments...
Show Resources