NOV 15, 2018 7:00 AM PST

Use of stem-cell derived endothelial cells for disease relevant cell modeling and drug discovery

Speakers
  • Senior Scientist, Roche
    Biography
      Filip Roudnicky is a senior scientist in the disease relevant cellular assay team in chemical biology at Roche. His is responsible for genome editing for disease modeling and for CRISPR/Cas9 genetic screens. He is also expert on disease relevant cellular assays involving endothelial cells. Filip started his career with a PhD in a lab of Prof. M. Detmar at ETH Zurich. He studied tumor angiogenesis of invasive bladder carcinoma and identified several biomarkers and molecular targets on tumor-associated blood vessels of bladder cancer. As a guest research scientist he has worked, in RIKEN Yokohama, Japan, under Dr. Jay W. Shin, on induced-neuronal stem cells and RNA-sequencing analysis. He has been a postdoctoral fellow in Roche and Harvard with the lab of Prof. C. Cowan developing an in vitro model of retinal endothelial cells.

    Abstract:

    The use of human pluripotent stem cells (hPSCs) for in vitro disease modeling is limited by the lack of robust and efficient protocols for the differentiation of relevant adult cell types. Previously, we have reported a method to generate vascular endothelial cells from hPSCs (Patsch et al. Nat Cell Biol. 2015). This novel and robust protocol in conjunction with use of programmed nucleases allows generation of relevant endothelilal cell disease models. We will show examples of modeling high-barrier resistance endothelial cells in vitro, by generation of CLDN5 reporter and use of chemical profiling, and modeling the effect of a severe metabolic genetic disease (AKT2 loss-of-function or dominant active mutation) on endothelial cells.


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