Mark received his Ph.D. in Biochemistry from the University of Rochester and his B.S. in Biology from the University of Buffalo.
An ideal microbiome profiling method would produce strain level taxonomic classification of organisms and their relative quantities. We demonstrate proof of principle experiments that sequencing methods using amplicons derived from rRNA gene clusters can be designed to differentiate between known strains of Clostridium difficile, a common hospital acquired pathogen. Obtaining consistent and reproducible results depends on overcoming challenges in microbial lysis, amplicon design, PCR primer degeneracy, sample multiplexing technology, and sequence database analysis. Novel approaches developed for each of these challenges are discussed.
University
Research And Development
Gene Expression
Cell Biology
Therapeutics
Biotechnology
Biomedical Research
Research
Molecular Biology
Molecular Genetics
Immunology
Gene Sequencing
Cloning
Cancer Research
Next-Generation Sequencing
Asia67%
North America33%
Website Visitors100%
Clinical Laboratory Scientist33%
Educator/Faculty33%
Scientist33%
Academic Institution33%
Clinical Laboratory33%
Research Institute33%