MAY 10, 2018 10:30 AM PDT

Strain Level Microbiome Profiling Using rRNA Gene Amplicons

C.E. Credits: CEU
Speaker

Abstract

An ideal microbiome profiling method would produce strain level taxonomic classification of organisms and their relative quantities. We demonstrate proof of principle experiments that sequencing methods using amplicons derived from rRNA gene clusters can be designed to differentiate between known strains of Clostridium difficile, a common hospital acquired pathogen. Obtaining consistent and reproducible results depends on overcoming challenges in microbial lysis, amplicon design, PCR primer degeneracy, sample multiplexing technology, and sequence database analysis. Novel approaches developed for each of these challenges are discussed.


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MAY 10, 2018 10:30 AM PDT

Strain Level Microbiome Profiling Using rRNA Gene Amplicons

C.E. Credits: CEU

Specialty

University

Research And Development

Gene Expression

Cell Biology

Therapeutics

Biotechnology

Biomedical Research

Research

Molecular Biology

Molecular Genetics

Immunology

Gene Sequencing

Cloning

Cancer Research

Next-Generation Sequencing

Geography

Asia67%

North America33%

Registration Source

Website Visitors100%

Job Title

Clinical Laboratory Scientist33%

Educator/Faculty33%

Scientist33%

Organization

Academic Institution33%

Clinical Laboratory33%

Research Institute33%


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