Chief Scientific Officer, Co-Founder, Shoreline Biome, LLC
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MAY 10, 2018 10:30 AM PDT
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Strain Level Microbiome Profiling Using rRNA Gene Amplicons
Presented at:
Genetics and Genomics 2018
Speaker
Abstract
An ideal microbiome profiling method would produce strain level taxonomic classification of organisms and their relative quantities. We demonstrate proof of principle experiments that sequencing methods using amplicons derived from rRNA gene clusters can be designed to differentiate between known strains of Clostridium difficile, a common hospital acquired pathogen. Obtaining consistent and reproducible results depends on overcoming challenges in microbial lysis, amplicon design, PCR primer degeneracy, sample multiplexing technology, and sequence database analysis. Novel approaches developed for each of these challenges are discussed.
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MAY 10, 2018 10:30 AM PDT
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Strain Level Microbiome Profiling Using rRNA Gene Amplicons
Presented at:
Genetics and Genomics 2018
Specialty
University
Research And Development
Gene Expression
Cell Biology
Therapeutics
Biotechnology
Biomedical Research
Research
Molecular Biology
Molecular Genetics
Immunology
Gene Sequencing
Cloning
Cancer Research
Next-Generation Sequencing
Geography
Asia67%
North America33%
Registration Source
Website Visitors100%
Job Title
Clinical Laboratory Scientist33%
Educator/Faculty33%
Scientist33%
Organization
Academic Institution33%
Clinical Laboratory33%
Research Institute33%
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