AUG 21, 2019 8:00 AM PDT

Streamlined translation of protein biomarkers discovered using ion mobility MS to targeted high throughput QQQ quantitative methods (Postponed)

Sponsored by: Agilent
Speaker
  • Associate Professor - Florey Institute of Neuroscience and Mental Health-University of Melbourne
    Biography
      Blaine R. Roberts is an Associate Professor at the Florey Institute of Neuroscience and Mental Health-University of Melbourne. His research group focuses on using proteomics to understand Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. He has a particular interest in understanding the role of metals in biology and has developed new proteomic technologies to measure metalloproteins. Further his group is using proteomics to characterize new blood borne biomarkers for Alzheimer's and Parkinson's disease.

    Abstract

    DATE: August 21, 2019

    TIME:  8:00am PDT

    This event has been postponed until further notice. We will notify you as soon as a new presentation date is confirmed.

    There is a growing requirement for validation and translation of protein targets discovered using high resolution high mass accuracy mass spectrometry on to high throughput platforms like QQQ or ELISA. Here we show the use of automated sample processing with the Bravo AssayMAP for both discovery proteomics on the Agilent 6560 ion mobility-MS and targeted analysis on the Agilent 6495 QQQ. The workflow represents a key advance in the ability to transition from a discovery sample set of 10-100 samples to validation cohorts of 1,000’s of biofluid samples (e.g. CSF, blood and tissue).  We will discuss the use of the label free ion mobility mass spectrometry with a particularly emphasis on how to process ion-mobility data using an accurate mass, retention time and collisional cross section measurement approach with Skyline software.  A research application example for neurodegenerative disease will be discussed leading from discovery to final method with isotopically labelled internal standards all on the Agilent Jet Stream ion source and AdvanceBio columns.

     

    For Research Use Only. Not for use in diagnostic procedures.

     

    Webinars will be available for unlimited on-demand viewing after live event.

     


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