SEP 30, 2015 1:30 PM PDT

SuperSelective Primers for the Multiplex Quantitation of Rare Mutant Sequences Associated with Cancer

Speaker
  • Professor, Public Health Research Institute, New Jersey Medical School, Rutgers University
    Biography
      Fred Russell Kramer is Professor of Microbiology and Molecular Genetics at the New Jersey Medical School, and has been a Principal Investigator at the Public Health Research Institute for the past 25 years. He graduated from the University of Michigan in 1964 and received his doctorate from the Rockefeller University in 1969. He was on the faculty of the Department of Genetics and Development at Columbia University College of Physicians and Surgeons for 17 years and has been a Research Professor and Adjunct Professor in the Department of Microbiology at New York University School of Medicine for the past 24 years.

    Abstract

    A major goal of molecular diagnostics is to find a sensitive and specific means for detecting and quantifying DNA fragments from rare cancer cells (by virtue of an identifying somatic mutation) in a clinical sample containing DNA fragments from abundant normal cells. Such a sensitive technique would enable "liquid biopsies" of the DNA fragments released into blood plasma as a consequence of apoptosis and necrosis (irrespective of the location of the tumor), enabling an early determination of prognosis, propensity to metastasize, and sensitivity to different therapies, for each individual patient. The approach taken by our laboratory towards meeting this challenge is to design oligonucleotide primers for polymerase chain reaction (PCR) assays that are extraordinarily selective, initiating amplification on (mutant) sequences that are perfectly complementary to the primer, while virtually ignoring (wild-type) sequences that mismatch the primer sequence by a single-nucleotide difference.


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