MAR 16, 2016 07:30 AM PDT

The Benefits of Combining Optogenetics, Microscopy and Electrophysiology

Presented At Neuroscience
  • Product Manager for Microscopy Systems, Andor Technology
      Dr Geraint Wilde studied Pharmacology at the University of Leeds, before proceeding to postgraduate studies at the University of Southampton. After attaining a PhD in 1997 Geraint continued his interest in neuroscience with a post-doctoral position at the University of Warwick.

      Following this post, he then moved to the University of Liverpool to work in the laboratory of Professor Michael White, focusing on intracellular signalling and gene expression through live cell imaging. Geraint used microscopy through most of his research career, and having caught the imaging bug, decided to pursue a commercial career in this field.

      Having worked for a few companies selling microscopy products, Geraint joined Andor Technology in 2009. Since then he has moved into the role of Product Manager for Microscopy Systems, focusing on product development, applications, delivery to market and support.


    Optogenetics or optophysiology is a rapidly growing technique used across an ever broadening array of research fields. Investigators now genetically modify many signalling pathway elements to enable precise and spatially targeted light control of biology down to a single cell or sub-cellular level.  Neuroscience is probably the largest research field using targeted light in order to control neuronal excitation and so gain better insight into how nerve cells communicate, how they behave within the context of a network, and the impact on the whole model organism.
    This webinar will provide a brief background into what optogenetics is, and more specifically highlight some publications that combine microscopy with optogenetic manipulation.  This will demonstrate the power of these tools in neuroscience research.  We will then go on to show technology that enables the researcher to photo-stimulate optogenetically modified targets using microscopy, and the power of combining this with electrophysiology techniques in the combined technique of optophysiology.

    Learning Objectives
    • Benefits of combining microscopy with optogenetic manipulation
    • How to mix photo-stimulation with electrophysiology  to enable optophysiological studies.

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