MAY 04, 2016 10:00 AM PDT

The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification

Speaker
  • Research Scientist, Life Sciences Solutions Group, Thermo Fisher Scientific
    Biography
      Lina Mpanda MSc. is a research scientist in the Life Sciences Solutions Group at Thermo Fisher Scientific in Frederick, MD. While at Thermo Fisher scientific, Lina has served as technical lead for new product development efforts for products utilized in downstream processing of therapeutic proteins and is currently supporting development and post product launch efforts for products used in cell biology applications (upstream). Lina's work specifically focuses on analytical characterization and purification of therapeutic proteins. She received her Masters of Pharmaceutical Science degree from Northeastern University, Boston, MA and her undergraduate degree from Sacred Heart University, Fairfield, CT.

    Abstract
    The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system, and differences between CHO culture conditions, purification conditions may vary.  In this presentation, protocols are presented for high efficiency, scalable, supernatant purification using Protein A chromatography resins. Differences in purification conditions for each culture condition are noted and product quality is discussed.
     

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