The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification

The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system, and differences between CHO culture conditions, purification conditions may vary.  In this presentation, protocols are presented for high efficiency, scalable, supernatant purification using Protein A chromatography resins. Differences in purification conditions for each culture condition are noted and product quality is discussed.