MAR 17, 2020 7:00 AM PDT

Time-Resolved STED Microscopy

Sponsored by: Leica Microsystems
C.E. Credits: Florida CE P.A.C.E. CE
Speaker

Abstract
DATE:  March 17, 2020
TIME:  7:00am PT, 10:00am ET
 
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological progress, STED microscopy can now provide effective sub-diffraction spatial resolution, while preserving most of the useful aspects of fluorescence microscopy, such as optical sectioning, molecular specificity and sensitivity, and live-cell compatibility.
 
This seminar starts by presenting the basic principles of STED microscopy and the three significant conditions required for its implementation, specifically the spatial, spectral, and temporal conditions. Towards these conditions, the seminar introduces the initial limitations and problems of STED microscopy and how modern STED microscopy architectures have circumvented these limitations. In particular, the webinar shows how the high illumination intensity - required to achieve tens of nanometer spatial resolution - can be mitigated by time-resolved STED microscopy, namely by recording and analyzing the temporal dynamics of the fluorescence signal (i.e., the fluorescence lifetime). In this context, the seminar explains and compares the time-gated and, more recently, separation-of-photons-by-lifetime-tuning (SPLIT) STED microscopy implementations.
 
Learning Objectives:  
  • Spatial, Spectral and Temporal Conditions for STED microscopy
  • Mitigating high illumination intensity with time-resolved STED microscopy taking advantage of fluorescence lifetime analysis (FLIM)
  • Comparison of time-gated and Separation-of-Photons-by-Lifetime (SPLIT) STED applications

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
Loading Comments...
Show Resources