FEB 20, 2017 8:00 AM PST

Tips, hints and trouble shooting: optimization of your recombinant protein preparation workflow

Speaker
  • Product Manager - HyClone™ Cell Culture Research, GE Healthcare Life Sciences
    Biography
      QuangVu graduated from the University of Washington with a Chemistry degree and an MBA from the University of Maryland. Having over 10+ years in the biotech industry, he is responsible for HyClone's products in research.

    Abstract

    First established in the 1880’s, cell culture is the process by which cells are grown in a laboratory under controlled environment. The culturing of different kinds of cells supports scientific, technological and therapeutic developments in human and animal health. Technology has allowed the production of a diverse number of biologic compounds such as monoclonal antibodies, vaccines, and cytokines. Given their importance, cell culture strategies have been developed that address the environmental conditions and nutritional demands for optimal metabolism and proliferation of cell lines for different applications.  
     
    We will discuss vital elements to successful cell culture including the choice of medium that provides the necessary nutrients to the cells. Media range from classical formulations to those designed to promote cell growth that are of animal-free origin. Media formulations also include those that can promote cell growth in the absence of serum supplementation and those that are chemically defined. GE Healthcare provides such media for many of the cell culture dependent biotechnology applications.
     
    While media is an important component of successful cell culture, reliable results and optimal production can only be achieved while maintaining excellent control over the process of cell growth, which is susceptible to contamination by various sources. We review critical aspects of prevention with simple techniques and good laboratory practice.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    APR 19, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 19, 2021 8:00 AM PDT
    Date: Apri 19, 2021 Time: 8:00am PDT, 5:00pm CEST Since the inception of modern biological research, BioSafety has evolved into a critical consideration in Good Laboratory Practices (GLP). Wi...
    FEB 20, 2017 8:00 AM PST

    Tips, hints and trouble shooting: optimization of your recombinant protein preparation workflow


    Specialty

    Cancer Research

    Pcr/rt-Pcr/real-Time Pcr

    Gene Expression

    Chemistry

    Cell Biology

    Bioinformatics

    Protein Chemistry

    Biology

    Genetics

    Chromatography

    Cell Culture

    Biomedical Engineering

    Antibodies

    Molecular Biology

    Drug Discovery

    Geography

    Europe45%

    North America27%

    Asia18%

    South America9%

    Registration Source

    Website Visitors100%

    Job Title

    Scientist44%

    Research Scientist22%

    Executive11%

    Educator/Faculty11%

    Post Doc11%

    Organization

    Research Institute9%

    Biotech Company9%

    Contract Research Organization (Cro)9%

    Other9%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more