MENU
FEB 20, 2017 8:00 AM PST

Tips, hints and trouble shooting: optimization of your recombinant protein preparation workflow

Speaker
  • Product Manager - HyClone™ Cell Culture Research, GE Healthcare Life Sciences
    Biography
      QuangVu graduated from the University of Washington with a Chemistry degree and an MBA from the University of Maryland. Having over 10+ years in the biotech industry, he is responsible for HyClone's products in research.

    Abstract

    First established in the 1880’s, cell culture is the process by which cells are grown in a laboratory under controlled environment. The culturing of different kinds of cells supports scientific, technological and therapeutic developments in human and animal health. Technology has allowed the production of a diverse number of biologic compounds such as monoclonal antibodies, vaccines, and cytokines. Given their importance, cell culture strategies have been developed that address the environmental conditions and nutritional demands for optimal metabolism and proliferation of cell lines for different applications.  
     
    We will discuss vital elements to successful cell culture including the choice of medium that provides the necessary nutrients to the cells. Media range from classical formulations to those designed to promote cell growth that are of animal-free origin. Media formulations also include those that can promote cell growth in the absence of serum supplementation and those that are chemically defined. GE Healthcare provides such media for many of the cell culture dependent biotechnology applications.
     
    While media is an important component of successful cell culture, reliable results and optimal production can only be achieved while maintaining excellent control over the process of cell growth, which is susceptible to contamination by various sources. We review critical aspects of prevention with simple techniques and good laboratory practice.


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    DEC 03, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 03, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: December 3, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Scientific cameras used in applications such as light sheet microscopy and calcium/voltage imaging put a large emphasis on high speed...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Loading Comments...
    Show Resources
    Attendees
    • See more