APR 30, 2020 9:00 AM PDT

Tools and methods for CRISPR-Cas9 screening for functional genomics and drug discovery

Speaker
  • Senior Product Manager for Functional Genomics, Thermo Fisher Scientific
    Biography
      Louise has 15 years of experience in product management, marketing, and technical sales of reagents and tools for gene modulation, encompassing primarily RNA interference and CRISPR-Cas9.
      She received her Masters Degree in Molecular Biology & Genetics from Northwestern University.

    Abstract
    DATE:  April 30, 2020
    TIME:   9:00am PT, 12:00pm ET
     
    Loss-of-function studies are an established method for uncovering a gene’s role in a biological pathway or disease state. The advent of CRISPR-Cas9 for gene knockout has given researchers a powerful tool for single-gene studies as well as high-throughput screening applications. Some experimental systems and assays demand solutions that enable long-term phenotypic readout, delivery into difficult-to-transfect cells, and the ability to easily move from pooled to arrayed screening reagents. The Invitrogen™ lentiviral guide RNA portfolio offers a toolset to achieve these goals. We will additionally present a recent case study of how a screen with LentiArray™ gRNA reagents has led to a potential human cancer therapeutic.
     
    Learning Objectives:
    • Guidelines to determine the CRISPR-Cas9 reagents that are best suited for your experiment
    • The importance of proper controls and assay optimization when using a lentiviral CRISPR-Cas9 screening approach
    • How arrayed lentiviral CRISPR sgRNA enable long-term assays for phenotypic screening 
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

     


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