APR 30, 2020 9:00 AM PDT

Tools and methods for CRISPR-Cas9 screening for functional genomics and drug discovery

Speaker

Abstract
DATE:  April 30, 2020
TIME:   9:00am PT, 12:00pm ET
 
Loss-of-function studies are an established method for uncovering a gene’s role in a biological pathway or disease state. The advent of CRISPR-Cas9 for gene knockout has given researchers a powerful tool for single-gene studies as well as high-throughput screening applications. Some experimental systems and assays demand solutions that enable long-term phenotypic readout, delivery into difficult-to-transfect cells, and the ability to easily move from pooled to arrayed screening reagents. The Invitrogen™ lentiviral guide RNA portfolio offers a toolset to achieve these goals. We will additionally present a recent case study of how a screen with LentiArray™ gRNA reagents has led to a potential human cancer therapeutic.
 
Learning Objectives:
  • Guidelines to determine the CRISPR-Cas9 reagents that are best suited for your experiment
  • The importance of proper controls and assay optimization when using a lentiviral CRISPR-Cas9 screening approach
  • How arrayed lentiviral CRISPR sgRNA enable long-term assays for phenotypic screening 
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 


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