SEP 10, 2019 9:00 AM PDT

Not Your Typical Flow Cytometry Panel Design Webinar: A tool kit for selecting antibodies with the complete panel in mind



DATE: September 10, 2019
TIME: 9:00am PDT

After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is antibody panel design.   Optimizing a multicolor immunophenotyping panel is often described as an iterative, time consuming process.  The end goal of this process is to overcome the loss of sensitivity that occurs when combining fluorescent dyes for multicolor detection.  A challenge is that the application of panel design principles requires optimization of multiple variables simultaneously, empirically trading off sensitivity due to marker co-expression and inherent instrument detection characteristics.

In this webinar we will have a dynamic discussion between leaders of our research flow cytometry platform from both the assay development and the instrumentation development perspective. In the first part of this webinar we describe a practical step-wise method that organizes panel design principles in an easily understand diagram.  In the second part of the webinar we will use these principles to give an understanding of how new flow cytometry technology facilitates immunophenotyping experiments.  Using flow cytometry data we’ll compare the outcomes of panels with varying degrees of optimization as assessed by panel design principles.

Learning Objectives:

  • Learn why it isn’t always advantageous to use the brightest dyes for every marker
  • Learn how to incorporate instrument characteristics into your panel design for maximum sensitivity to detect low abundant and rare populations
  • Demonstrate a powerful concept for applying panel design best practices that can help novices to advanced flow cytometrists design and/or communicate their panel choices
  • See some real-world examples demonstrating how new technology is facilitating multicolor applications


Webinars will be available for unlimited on-demand viewing after live event.


LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 14, 2021 7:00 AM PDT
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
APR 21, 2021 5:00 PM CEST
APR 21, 2021 5:00 PM CEST
Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
SEP 28, 2021 9:00 AM PDT
SEP 28, 2021 9:00 AM PDT
Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
SEP 10, 2019 9:00 AM PDT

Not Your Typical Flow Cytometry Panel Design Webinar: A tool kit for selecting antibodies with the complete panel in mind



Molecular Biology

Cell Biology

Cancer Research

Cell Culture



Flow Cytometry

Cancer Therapeutics

Clinical Research

Laboratory Testing

Molecular Diagnostics


Clinical Diagnostics

Cancer Diagnostics


North America60%



South America2%



Registration Source

Website Visitors53%

Email Promotions35%

WAVE Newsletter Promotion6%

Client/Sponsor Promotions3%

Trending Newsletter Promotion2%


Job Title

Research Scientist14%



Medical Laboratory Technician10%

Facility/Department Manager10%


Lab Management7%


Post Doc5%

Medical Doctor/Specialist4%

Clinical Laboratory Scientist4%







Academic Institution24%


Biotech Company10%

Clinical Laboratory8%

Research Institute7%

Life Science Company6%

Pharmaceutical Company5%

Medical School3%


Medical Center3%

Contract Research Organization (Cro)2%


Medical Device Company1%

Non-Profit Organization1%


Show Resources
Loading Comments...
Show Resources