SEP 10, 2019 09:00 AM PDT

Not Your Typical Flow Cytometry Panel Design Webinar: A tool kit for selecting antibodies with the complete panel in mind

Speakers
  • Sr. Marketing Manager - Beckman Coulter Life Sciences
    Biography
      Maria Gentile manages the CytoFLEX Flow Cytometry Platform for Beckman Coulter Life Sciences. She has a keen interest in the cutting-edge research aimed at the understanding of the role of extracellular vesicles in health and disease as well as their potential in therapeutics. Additionally, Maria follows trends in flow cytometry detection technologies with potential for advancing the science while maintaining ease of use as a priority.
      Maria is a molecular biologist by training and received her Master of Science in Applied Molecular Biology from the University of Wisconsin-Parkside in 1994. She spent 12 years as a research scientist making contributions to product development for infectious disease, and basic research in the areas of pharmacogenetics and cancer biology. Maria is co-author on a patent granted for Amplification Based Polymorphism Detection and has filed a patent for Self-Complementary Molecular Probe.
      Prior to working for Beckman Coulter, Maria supported Sales and Applications for Bio-Rad Laboratories in both western Canada and the southwest US. She joined the technical sales group of Beckman Coulter in 2009 with responsibility for western Canada. In 2016, she transferred to the strategic marketing role to bring her deep knowledge of investigator's needs and commitment to customer satisfaction to guide the future product vision of this highly successful product line.
    • Sr. Marketing Manager - Beckman Coulter Life Sciences
      Biography
        Michael Kapinsky has a strategic marketing role at Beckman Coulter's Flow Cytometry division. As a member of the global business unit, Michael owns responsibility for the clinical research product line branded as DURAClone and contributes to Beckman Coulter's strategic clinical research initiative. Michael joined Beckman Coulter in 2002. Michael is following trends and future directions in biomedicine with an interest in novel therapeutic approaches based on immune modulation and targeting as well as on "living drugs". He leads the development of ready-to-use antibody panels for phenotypic and functional characterization of primary and genetically modified human cells.
        Upon obtaining his PhD at the Institute of Laboratory Medicine of the University of Regensburg/Germany he started as a field application specialist, tailoring multicolor flow cytometry applications to meet a diversity of needs in different environments, from basic research to regulated laboratories. Michael was granted patents in US and EU for a novel antibody panel design method. He continues to provide substantial technical support to international clinical research studies such as The ONE Study (www.onestudy.org). He was granted the Danaher "Excellence in Innovation" award for his contributions to the development of a novel diagnostic method for infectious diseases.

      Abstract:

      DATE: September 10, 2019
      TIME: 9:00am PDT

      After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is antibody panel design.   Optimizing a multicolor immunophenotyping panel is often described as an iterative, time consuming process.  The end goal of this process is to overcome the loss of sensitivity that occurs when combining fluorescent dyes for multicolor detection.  A challenge is that the application of panel design principles requires optimization of multiple variables simultaneously, empirically trading off sensitivity due to marker co-expression and inherent instrument detection characteristics.

      In this webinar we will have a dynamic discussion between leaders of our research flow cytometry platform from both the assay development and the instrumentation development perspective. In the first part of this webinar we describe a practical step-wise method that organizes panel design principles in an easily understand diagram.  In the second part of the webinar we will use these principles to give an understanding of how new flow cytometry technology facilitates immunophenotyping experiments.  Using flow cytometry data we’ll compare the outcomes of panels with varying degrees of optimization as assessed by panel design principles.

      Learning Objectives:

      • Learn why it isn’t always advantageous to use the brightest dyes for every marker
      • Learn how to incorporate instrument characteristics into your panel design for maximum sensitivity to detect low abundant and rare populations
      • Demonstrate a powerful concept for applying panel design best practices that can help novices to advanced flow cytometrists design and/or communicate their panel choices
      • See some real-world examples demonstrating how new technology is facilitating multicolor applications

       

      Webinars will be available for unlimited on-demand viewing after live event.

       

      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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