DATE: September 10, 2019
TIME: 9:00am PDT
After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is antibody panel design. Optimizing a multicolor immunophenotyping panel is often described as an iterative, time consuming process. The end goal of this process is to overcome the loss of sensitivity that occurs when combining fluorescent dyes for multicolor detection. A challenge is that the application of panel design principles requires optimization of multiple variables simultaneously, empirically trading off sensitivity due to marker co-expression and inherent instrument detection characteristics.
In this webinar we will have a dynamic discussion between leaders of our research flow cytometry platform from both the assay development and the instrumentation development perspective. In the first part of this webinar we describe a practical step-wise method that organizes panel design principles in an easily understand diagram. In the second part of the webinar we will use these principles to give an understanding of how new flow cytometry technology facilitates immunophenotyping experiments. Using flow cytometry data we’ll compare the outcomes of panels with varying degrees of optimization as assessed by panel design principles.
- Learn why it isn’t always advantageous to use the brightest dyes for every marker
- Learn how to incorporate instrument characteristics into your panel design for maximum sensitivity to detect low abundant and rare populations
- Demonstrate a powerful concept for applying panel design best practices that can help novices to advanced flow cytometrists design and/or communicate their panel choices
- See some real-world examples demonstrating how new technology is facilitating multicolor applications
Webinars will be available for unlimited on-demand viewing after live event.
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.