NOV 27, 2018 1:00 AM PST

Understand the rationale behind the Q-TOF LC/MS that stably sustains sub-ppm mass accuracy.

Sponsored by: Shimadzu
Speaker
  • MS Application Specialist, Analytical & Measuring Instruments Division, Shimadzu Corporation
    Biography
      Ash Toyama has 10 years of proteomics research experience. His areas of expertise include not only proteomics but also quantitative mass spectrometry with particular focus on bioanalysis, metabolomics and endocrinology. At Shimadzu Corporation he played roles in academic research collaborations, application development and specification development of mass spectrometers. Ash holds a Master of Science degree in Biochemistry from University of Cambridge, UK, and was granted a Ph.D. degree in Biological Sciences from University of Tokyo for his research on clinical proteomics.

    Abstract

    DATE: November 27, 2018
    TIME: 1:00am PT

    High-resolution, accurate-mass (HRAM) mass spectrometers (MS) are a class of MS instrumentation with capability to resolve complex sample matrix and to allow identification of compounds by measuring their accurate masses. In particular, those coupled to liquid chromatograph (HRAM LC/MS) have been used extensively for structural elucidation of unknown compounds primarily in chemical industry (impurity analysis) and biological researches (proteomics, metabolomics, lipidomics), but also increasingly in forensic science, food safety and environmental testing. Given the recent reduction of instrumentational cost, it will not take many more years until HRAM analysis becomes a versatile tool for all industries and researches. 

    The key performance attribute underlying all application fields is mass measurement accuracy (MMA) as it affords molecular specificity and reduction of false positive results. MMA is given in the specification of HRAM instruments in parts-per-million (ppm), frequently stating a result of a set of representative measurements e.g. “<1 ppm RMS”. However, this by itself does not convey how repeatably and reproducibly this MMA can be achieved. 

    The webinar describes various metrics that are relevant to statistical interpretation of MMA, which explain why mass resolution of 30,000 is more than sufficient for mass measurement at sub-ppm accuracy. Using the Shimadzu LCMS-9030 quadrupole time-of-flight MS, the rationale and strength of instrument stability for consistently achieving sub-ppm MMA has been demonstrated in small molecules.

    Learning objectives:

    • Discuss how various parameters affect the accuracy of mass measurement
    • Demonstration of mass drift caused by temperature change
    • Learn how to assess whether a HRAM MS is practically capable of measuring at sub-ppm mass accuracy

    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    APR 20, 2021 11:00 AM PDT
    C.E. CREDITS
    APR 20, 2021 11:00 AM PDT
    Date: April 20, 2021 Time: 11:00am (PST), 2:00pm (EST) Identification and confirmation of microbial isolates is crucial in the analysis workflow, providing decision-makers the information...
    APR 19, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 19, 2021 8:00 AM PDT
    Date: Apri 19, 2021 Time: 8:00am PDT, 5:00pm CEST Since the inception of modern biological research, BioSafety has evolved into a critical consideration in Good Laboratory Practices (GLP). Wi...
    NOV 27, 2018 1:00 AM PST

    Understand the rationale behind the Q-TOF LC/MS that stably sustains sub-ppm mass accuracy.

    Sponsored by: Shimadzu

    Specialty

    Biotechnology

    Chemistry

    Molecular Biology

    Clinical Research

    Biochemistry

    Cancer Research

    Assay Development

    Clinical Diagnostics

    Laboratory Testing

    Medicine

    Genetics

    Cell Biology

    Diagnostics

    Drug Discovery

    Clinical Chemistry

    Geography

    Asia38%

    North America23%

    Europe21%

    South America11%

    Africa5%

    Oceania1%

    Registration Source

    Website Visitors88%

    Email Promotions7%

    WAVE Newsletter Promotion4%

    Social Media Promotions1%

    Job Title

    Facility/Department Manager20%

    Research Scientist11%

    Student11%

    Educator/Faculty9%

    Scientist9%

    Marketing/Sales7%

    Executive7%

    Engineer5%

    Medical Doctor/Specialist5%

    Lab Management4%

    Clinical Laboratory Scientist4%

    Chemist3%

    Medical Laboratory Technician3%

    Post Doc1%

    Organization

    Academic Institution18%

    Distribution/sales12%

    Research Institute8%

    Biotech Company7%

    Hospital6%

    Industrial Company6%

    Pharmaceutical Company5%

    Government3%

    Clinical Laboratory3%

    Life Science Company3%

    Medical School2%

    Medical Center2%

    Consultant1%

    Ambulatory Care1%

    Other14%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more