FEB 06, 2014 06:00 AM PST

Use of Dried Blood Spot Samples in Serological Testing of Rodents

Speakers
  • Head of Serology, IDEXX BioResearch
    Biography

       
      Matthew H. Myles, DVM, PhD, DACLAM is a comparative immunologist with over 10 years' experience in defining the immunologic response to infectious disease. Dr. Myles serves as Head of Serology where he oversees production and quality control of serologic reagents and assays, supervises serology personnel, develops new serologic assays, and provides final approval for case reports.
       

    Abstract:

    The utility of dried blood spot (DBS) sampling technology for rodent colony health monitoring was investigated using a two-tiered approach. In the first approach, groups of mice and rats were experimentally infected. Mice were inoculated with epizootic diarrhea of infant mice virus (EDIM), mouse hepatitis virus (MHV), murine norovirus (MNV), mouse parvovirus (MPV), or Theiler's murine encephalomyelitis virus (TMEV). Rats were inoculated with Kilham rat virus (KRV) or rat theilovirus (RTV). Paired DBS and serum samples were collected at 2, 4, 6, and 12 weeks post-inoculation. In the second approach, designed to evaluate real-world conditions, over 1000 mouse and rat paired DBS and serum samples were collected from collaborating North American institutions. Dried blood spot and serum samples were evaluated by multiplex fluorescent immunoassay (MFI) against a comprehensive panel of infectious disease assays and data were compared for magnitude of MFI signal, signal to noise ratio, correlation, and stability. Results from these studies demonstrated that the magnitude of the mean MFI signal from the DBS samples was equal to or higher than the corresponding serum sample. Moreover, the assay signal-to-noise ratio from the DBS samples was equal to or higher than the corresponding serum samples. Evaluation of a large cohort of paired clinical DBS and serum samples showed 100% diagnostic correlation and improved specificity. Finally, stability studies showed no degradation of MFI signal when DBS samples were protected from humidity and stored at room temperature or 4°C for up to 14 days or when stored at -20°C for up to 3 months, compared to controls. A moderate decrease in MFI signal was encountered when samples were stored at 37°C in 95% humidity for 24 hours; however, all samples remained diagnostically positive.


    Show Resources
    You May Also Like
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    JUL 31, 2019 09:00 AM PDT
    C.E. CREDITS
    JUL 31, 2019 09:00 AM PDT
    DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
    Loading Comments...
    Show Resources