FEB 06, 2014 6:00 AM PST

Use of Dried Blood Spot Samples in Serological Testing of Rodents

Speaker

Abstract

The utility of dried blood spot (DBS) sampling technology for rodent colony health monitoring was investigated using a two-tiered approach. In the first approach, groups of mice and rats were experimentally infected. Mice were inoculated with epizootic diarrhea of infant mice virus (EDIM), mouse hepatitis virus (MHV), murine norovirus (MNV), mouse parvovirus (MPV), or Theiler's murine encephalomyelitis virus (TMEV). Rats were inoculated with Kilham rat virus (KRV) or rat theilovirus (RTV). Paired DBS and serum samples were collected at 2, 4, 6, and 12 weeks post-inoculation. In the second approach, designed to evaluate real-world conditions, over 1000 mouse and rat paired DBS and serum samples were collected from collaborating North American institutions. Dried blood spot and serum samples were evaluated by multiplex fluorescent immunoassay (MFI) against a comprehensive panel of infectious disease assays and data were compared for magnitude of MFI signal, signal to noise ratio, correlation, and stability. Results from these studies demonstrated that the magnitude of the mean MFI signal from the DBS samples was equal to or higher than the corresponding serum sample. Moreover, the assay signal-to-noise ratio from the DBS samples was equal to or higher than the corresponding serum samples. Evaluation of a large cohort of paired clinical DBS and serum samples showed 100% diagnostic correlation and improved specificity. Finally, stability studies showed no degradation of MFI signal when DBS samples were protected from humidity and stored at room temperature or 4°C for up to 14 days or when stored at -20°C for up to 3 months, compared to controls. A moderate decrease in MFI signal was encountered when samples were stored at 37°C in 95% humidity for 24 hours; however, all samples remained diagnostically positive.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
APR 01, 2021 8:00 AM PDT
C.E. CREDITS
APR 01, 2021 8:00 AM PDT
Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
FEB 24, 2021 10:00 AM PST
C.E. CREDITS
FEB 24, 2021 10:00 AM PST
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
MAY 18, 2021 8:00 AM PDT
C.E. CREDITS
MAY 18, 2021 8:00 AM PDT
Date: May 18, 2021 Time: 8:00am (PST) The global pandemic has caused an increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provide qu...
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
JAN 21, 2021 8:00 AM PST
JAN 21, 2021 8:00 AM PST
Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
FEB 06, 2014 6:00 AM PST

Use of Dried Blood Spot Samples in Serological Testing of Rodents


Specialty

Antibodies

Virology

Immunology

Personalized Medicine

Dna Sequencing

Immunity

Clinical Diagnostics

Cancer Diagnostics

Immunotherapy

Bioinformatics

Flow Cytometry

Immuno-Oncology

Gene Expression

Big Data

Dna

Geography

North America50%

Europe50%

Registration Source

Website Visitors100%

Job Title

Student50%

Medical Laboratory Technician50%

Organization

Pharmaceutical Company50%

Academic Institution50%


Show Resources
Loading Comments...
Show Resources
Attendees
  • See more