FEB 06, 2014 06:00 AM PST

Use of Dried Blood Spot Samples in Serological Testing of Rodents

Speakers
  • Head of Serology, IDEXX BioResearch
    Biography

       
      Matthew H. Myles, DVM, PhD, DACLAM is a comparative immunologist with over 10 years' experience in defining the immunologic response to infectious disease. Dr. Myles serves as Head of Serology where he oversees production and quality control of serologic reagents and assays, supervises serology personnel, develops new serologic assays, and provides final approval for case reports.
       

    Abstract:

    The utility of dried blood spot (DBS) sampling technology for rodent colony health monitoring was investigated using a two-tiered approach. In the first approach, groups of mice and rats were experimentally infected. Mice were inoculated with epizootic diarrhea of infant mice virus (EDIM), mouse hepatitis virus (MHV), murine norovirus (MNV), mouse parvovirus (MPV), or Theiler's murine encephalomyelitis virus (TMEV). Rats were inoculated with Kilham rat virus (KRV) or rat theilovirus (RTV). Paired DBS and serum samples were collected at 2, 4, 6, and 12 weeks post-inoculation. In the second approach, designed to evaluate real-world conditions, over 1000 mouse and rat paired DBS and serum samples were collected from collaborating North American institutions. Dried blood spot and serum samples were evaluated by multiplex fluorescent immunoassay (MFI) against a comprehensive panel of infectious disease assays and data were compared for magnitude of MFI signal, signal to noise ratio, correlation, and stability. Results from these studies demonstrated that the magnitude of the mean MFI signal from the DBS samples was equal to or higher than the corresponding serum sample. Moreover, the assay signal-to-noise ratio from the DBS samples was equal to or higher than the corresponding serum samples. Evaluation of a large cohort of paired clinical DBS and serum samples showed 100% diagnostic correlation and improved specificity. Finally, stability studies showed no degradation of MFI signal when DBS samples were protected from humidity and stored at room temperature or 4°C for up to 14 days or when stored at -20°C for up to 3 months, compared to controls. A moderate decrease in MFI signal was encountered when samples were stored at 37°C in 95% humidity for 24 hours; however, all samples remained diagnostically positive.


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 02, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 02, 2018 08:00 AM PDT
    Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    Loading Comments...