NOV 07, 2019 10:00 AM PST

Akoya Biosciences Webinar Series: Validating Multiplexed Antibody Panels for Pancreatic Research Applications

Sponsored by: Akoya Biosciences
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Staff Scientist, Powers & Brissova Research Group, Vanderbilt University Medical Center, USA
    Biography
      Diane completed her dissertation research on the role of pancreatic endothelial cells in the islet microenvironment, and is now leading efforts to characterize human pancreas development using tissue samples from organ donors aged birth to ten years. In addition, she is the Co-Scientific Director of Pancreatlas™, an online image resource that houses reference datasets from human pancreas samples (https://pancreatlas.org/).
    • Technical Application Scientist, Akoya Biosciences, Inc.
      Biography
        Grady is a Technical Application Scientist with experience in basic and translation pathology-based research, with a focus on assay development for detection and characterization of biomarkers in tissues and circulating tumor cells. Grady joined Akoya Biosciences with the acquisition of PerkinElmer's Quantitative Pathology Solutions business which includes flagship brands like the Vectra® and Mantra™ family of quantitative pathology systems, Opal™ multiplex IHC Staining Kits and Panels, and inForm® Tissue Analysis Software. Grady's most recent academic stint was from The Baylor College of Medicine, where he researched breast cancer metastasis as a postdoctoral associate.

      Abstract
      DATE:  November 7, 2019
      TIME:   10:00am PST, 1:00pm EST
       
      Studying the pathogenesis of diabetes requires detailed analysis of the pancreatic islet microenvironment and its numerous cell types, at the single-cell level. Profiling these complex cellular phenotypes is greatly enhanced by the application of highly multiplexed immunofluorescence technology that can also retain the spatial context of the entire tissue section.
       
      The CODEX® System is a compact, budget-friendly instrument that integrates with third-party fluorescence microscopes and images 40+ protein markers in a single tissue section. The platform is part of an end-to-end solution that also includes inventoried antibodies, reagents, and a software solution for visualizing cellular phenotypes. In the Powers and Brissova Research Group at Vanderbilt University, the CODEX platform is used to investigate unique cellular niches in the developing human pancreas, including describing the interactions between endocrine cells and neurovascular and immune compartments.
       
      In this webinar, Diane Saunders, a staff scientist in the Vanderbilt Diabetes Center, will describe the validation process for custom panels of CODEX antibodies using lightly fixed tissue samples. She will also share the preliminary results from her validation studies, where she used a multiplexed CODEX panel with a mix of preconjugated and custom-conjugated antibodies, to profile pancreatic islet development in neonatal human samples.
       
      Grady Carlson, a Technical Application Scientist at Akoya Biosciences, will introduce the CODEX technology and workflow and how it can be applied for biomarker discovery.
       
      Learning Objectives:
      • Outlining tips & tricks to validate highly multiplexed antibody panels for lightly-fixed pancreatic tissue
      • Demonstrating why spatially-resolved, multiplexed immunofluorescence is essential for tissue analysis and biomarker discovery
      • Investigating how the CODEX solution enables the simultaneous analysis of 40+ proteins in FFPE and fresh frozen tissue sections
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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