SEP 24, 2019 9:00 AM PDT

Validating single-cell RNA-seq data using Single-Cell Western blotting and RNAscope® technology

Speaker
  • Associate Professor, University of Texas at San Antonio
    Biography
      Dr. Hermann received his PhD in 2005 from the University of Kansas, performed postdoctoral training at the Magee-Womens Research Institute and started his lab at the University of Texas at San Antonio in 2011. The Hermann lab studies spermatogonial stem cells (SSCs), which are adult stem cells that produce sperm throughout life and are essential for male fertility. Dr. Hermann studies the mechanisms regulating SSC fate, SSCs fate specification during testis development, and potential SSC-based therapeutic strategies for male infertility. The Hermann lab has pioneered use of single-cell methods to help uncover novel pathways involved in SSC fate control and revolutionize understanding of spermatogenesis.

    Abstract

    DATE: September 24, 2019

    TIME: 9:00am PDT

    This webinar will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAscope single-molecule fluorescence RNA in situ hybridization (smFISH) technologies and present data from an ongoing study in the laboratory of Dr. Brian Hermann at the University of Texas at San Antonio where both platforms are being used to validate single-cell RNA-seq data. Dr. Hermann studies spermatogonial stem cells (SSCs), which are adult stem cells responsible for life-long sperm production in the testes of mammals. Single-cell RNA-seq studies from his laboratory identified substantial gene expression heterogeneity among undifferentiated spermatogonia (which include both SSCs and the progenitor spermatogonia that they produce) at the mRNA level. Results from ongoing validation studies in his laboratory will be presented. Dr. Hermann has used Milo SCW to confirm gene expression heterogeneity at the protein level among individual cells and RNAscope smFISH has validated heterogeneity at the mRNA level and facilitated spatial localization of cell subsets.

     

    Learning Objectives:

    • Describe how Milo Single-Cell Western blotting can provide protein-level validation of heterogeneity among individual cells uncovered by single-cell RNA-seq (scRNA-seq)

    • Identify how RNAscope smFISH can be used to validate scRNA-seq data at the mRNA level and enable spatial remapping to determine localization of cells with particular phenotypes.

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

      


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