JUL 15, 2021 9:00 AM PDT

Veranome Webinar Series: Highly Multiplexed Analysis of Mouse Neural Tissues

Sponsored by: Veranome Biosystems
Speaker

Abstract
Date:  July 15, 2021
Time: 9:00am (PDT),  12:00pm (EDT)
 
The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeBio’s Pisces workflow consists of 1) a novel assay for the massively parallel imaging >100s of RNA targets, 2) state-of-the-art imaging technology delivering sub-cellular resolution, and 3) a software suite for from custom panel design, experimental setup and data processing, as well as sophisticated visualization and statistical analytics of segmented cell results. The presentation includes detailed analysis of human and mouse tissues, including tumor samples, mouse neural tissues and organoids.
 
We enable researchers to deepen their understanding of diseases with high-quality data of how individual cells organize within tissue, using robust workflows, reliable imaging systems and without the need for extensive bioinformatics tools or infrastructure. In this study, we demonstrate the results with mouse liver and neural tissues, as well as the spatial organization of differing cell layers composing the structure of a newly harvested organoid. Initiating with a PFA-fixed tissue mounted in a proprietary flow cell, a targeted panel is used to localized individual RNA molecule within specific compartments within a cell. Through cyclic reagent addition and imaging, the barcoded panel enables the Pisces system to reveal and decode custom or pre-validated panels tied to specific disease states or biological functions. Individual cells are identified through advanced, AI-based segmentation algorithms, and individual RNA molecules are quantified within cell boundaries. The resulting matrix of individual cells, summarized RNA counts, coupled with X, Y and Z coordinates are used for statistical clustering and phenotyping, as well as spatial relationships characterizing the molecular and cellular niches and architecture. The Veranome system provides the first commercial system for high-content imaging and analysis of true single cell, spatial transcriptomics.
 
Learning Objectives
  • Novel method for highly multiplexed spatial RNA analysis
  • Sub-cellular resolution of the mouse brain for characterization of the tissue architecture
  • AI-based cell segmentation and statistical relationships of multiple cell types and functions
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 

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