SEP 10, 2015 04:00 AM PDT

WEBINAR: Visualizing & Tracking: Extracellular Vesicles Delivery and RNA Translation

Speakers
  • Assistant Professor, Institute of Biomedical Engineering, National Tsing-Hua University
    Biography
      Charles Lai received his B.Sc (2003) and Ph.D. (2010) from the University of British Columbia, Canada, where he studied the role of gap junctions and its analogues pannexins in glioma, under the guidance of Prof. Christian Naus. In 2010, he joined Profs. Xandra Breakefield and Bakhos Tannous' laboratories in Massachusetts General Hospital and Harvard Medical School as a Postdoctoral Fellow, and later in 2014 as an Instructor, to expand his research horizon in cell-to-cell communication, centering on extracellular vesicle (EV)-mediated communication between glioma and glioma-stromal cells. He developed one of the first noninvasive methods to monitor EVs and cargo in vitro and in vivo. He is a recipient of the Postdoctoral Fellowship from the Canadian Institutes of Health Research (CIHR). In 2015, Dr. Lai joined National Tsing Hua University (Hsinchu, Taiwan) as an Assistant Professor of the Institute of Biomedical Engineering. Dr. Lai's research focuses on tumor EV processes, imaging and therapeutic development.

    Abstract:

    The webinar will start at 4am Pacific Time, 7am Eastern Time, 1pm Central European Summer Time, 7pm China Standard Time

     

    Extracellular vesicles (EVs) including exosome and microvesicles are nanometer sized vesicles released by cells as a novel form of intercellular communication without direct cell-to-cell contacts, delivering lipids, cellular proteins, mRNAs and non-coding RNAs. Recent studies have shown that EV plays an important role in diseases, as well as been employed as a vehicle for targeted delivery of therapeutic genes/drugs. Accurate spatiotemporal assessment of nanosized EV delivery and cargo RNA translation, however, requires specific and robust imaging technologies. For fluorescent reporters conjugated to proteins enriched in EVs, labeling may be restricted to subpopulations of EVs, limiting their wider use to observe multiple EV types. Meanwhile, fluorescent dyes for EV lipid-labeling including PKH may not reflect true half-life of EVs and can be retained in association with other lipid entities for long periods, thus misguiding spatiotemporal assessment of EV dynamics, especially over extended periods. This webinar will discuss emerging methods developed for EV visualization, as well as findings made from employing these new technologies.

    Learning Objectives:

    • Discuss emerging methods developed for EV visualization
    • Understand findings made from employing new technologies developed for extracellular vesicle (EV) visualization

    Show Resources
    You May Also Like
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 02, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 02, 2018 08:00 AM PDT
    Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    Loading Comments...