MENU
SEP 10, 2015 4:00 AM PDT

WEBINAR: Visualizing & Tracking: Extracellular Vesicles Delivery and RNA Translation

Speaker
  • Assistant Professor, Institute of Biomedical Engineering, National Tsing-Hua University
    Biography
      Charles Lai received his B.Sc (2003) and Ph.D. (2010) from the University of British Columbia, Canada, where he studied the role of gap junctions and its analogues pannexins in glioma, under the guidance of Prof. Christian Naus. In 2010, he joined Profs. Xandra Breakefield and Bakhos Tannous' laboratories in Massachusetts General Hospital and Harvard Medical School as a Postdoctoral Fellow, and later in 2014 as an Instructor, to expand his research horizon in cell-to-cell communication, centering on extracellular vesicle (EV)-mediated communication between glioma and glioma-stromal cells. He developed one of the first noninvasive methods to monitor EVs and cargo in vitro and in vivo. He is a recipient of the Postdoctoral Fellowship from the Canadian Institutes of Health Research (CIHR). In 2015, Dr. Lai joined National Tsing Hua University (Hsinchu, Taiwan) as an Assistant Professor of the Institute of Biomedical Engineering. Dr. Lai's research focuses on tumor EV processes, imaging and therapeutic development.

    Abstract

    The webinar will start at 4am Pacific Time, 7am Eastern Time, 1pm Central European Summer Time, 7pm China Standard Time

     

    Extracellular vesicles (EVs) including exosome and microvesicles are nanometer sized vesicles released by cells as a novel form of intercellular communication without direct cell-to-cell contacts, delivering lipids, cellular proteins, mRNAs and non-coding RNAs. Recent studies have shown that EV plays an important role in diseases, as well as been employed as a vehicle for targeted delivery of therapeutic genes/drugs. Accurate spatiotemporal assessment of nanosized EV delivery and cargo RNA translation, however, requires specific and robust imaging technologies. For fluorescent reporters conjugated to proteins enriched in EVs, labeling may be restricted to subpopulations of EVs, limiting their wider use to observe multiple EV types. Meanwhile, fluorescent dyes for EV lipid-labeling including PKH may not reflect true half-life of EVs and can be retained in association with other lipid entities for long periods, thus misguiding spatiotemporal assessment of EV dynamics, especially over extended periods. This webinar will discuss emerging methods developed for EV visualization, as well as findings made from employing these new technologies.

    Learning Objectives:

    • Discuss emerging methods developed for EV visualization
    • Understand findings made from employing new technologies developed for extracellular vesicle (EV) visualization

    Show Resources
    You May Also Like
    AUG 18, 2020 10:00 AM PDT
    C.E. CREDITS
    AUG 18, 2020 10:00 AM PDT
    DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    DEC 03, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 03, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: December 3, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Scientific cameras used in applications such as light sheet microscopy and calcium/voltage imaging put a large emphasis on high speed...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    DEC 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    Loading Comments...
    Show Resources
    Attendees
    • See more