The webinar will start at 4am Pacific Time, 7am Eastern Time, 1pm Central European Summer Time, 7pm China Standard Time
Extracellular vesicles (EVs) including exosome and microvesicles are nanometer sized vesicles released by cells as a novel form of intercellular communication without direct cell-to-cell contacts, delivering lipids, cellular proteins, mRNAs and non-coding RNAs. Recent studies have shown that EV plays an important role in diseases, as well as been employed as a vehicle for targeted delivery of therapeutic genes/drugs. Accurate spatiotemporal assessment of nanosized EV delivery and cargo RNA translation, however, requires specific and robust imaging technologies. For fluorescent reporters conjugated to proteins enriched in EVs, labeling may be restricted to subpopulations of EVs, limiting their wider use to observe multiple EV types. Meanwhile, fluorescent dyes for EV lipid-labeling including PKH may not reflect true half-life of EVs and can be retained in association with other lipid entities for long periods, thus misguiding spatiotemporal assessment of EV dynamics, especially over extended periods. This webinar will discuss emerging methods developed for EV visualization, as well as findings made from employing these new technologies.