JUN 21, 2016 8:00 AM PDT

Combining Aggregate Determination with Light Scattering Detection: More Data, More Easily

Speaker
  • Senior Applications Chemist, Agilent Technologies
    Biography
      I joined the Agilent Biocolumns Applications Team in August 2011, with many years experience in all kinds of LC: reversed phase, ion exchange, size exclusion, glycan analysis (HILIC) amongst others. I enjoy method development, technical support and troubleshooting and I have a "hands on" attitude to problem solving !

    Abstract
    Size Exclusion Chromatography is the method of choice for quantification of aggregates in Biotherapeutic samples using a UV detector for determining concentration.  However the addition of a light scattering detector capable of both dynamic and static light scattering measurements gives significant benefits.  Absolute molecular weight determination with enhanced light scattering response ensures that dimers are clearly identified, through both absolute molecular weight measurement and the enhanced response afforded by the LS detector towards higher molecular weight species.  Incorporating dynamic light scattering allows hydrodynamic radius determination providing the possibility of detecting conformational differences.  Furthermore, with the advent of antibody-drug conjugates (ADCs) where non-specific interactions are more likely to cause retention time differences, the light scattering detector provides greater reassurance and confidence in analytical data.  Join the Agilent experts for this discussion on the AdvanceBio and Agilent Infinity II approach to this area of application.  An open Q&A session on size exclusion in general will follow the talk.

    For Research Use Only. Not for use in diagnostic procedures.

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