JUN 21, 2016 08:00 AM PDT

Combining Aggregate Determination with Light Scattering Detection: More Data, More Easily

SPONSORED BY: Agilent Genomics, Agilent Genomics
C.E. CREDITS: P.A.C.E. CE
Speakers
  • Senior Applications Chemist, Agilent Technologies
    Biography
      I joined the Agilent Biocolumns Applications Team in August 2011, with many years experience in all kinds of LC: reversed phase, ion exchange, size exclusion, glycan analysis (HILIC) amongst others. I enjoy method development, technical support and troubleshooting and I have a "hands on" attitude to problem solving !

    Abstract:
    Size Exclusion Chromatography is the method of choice for quantification of aggregates in Biotherapeutic samples using a UV detector for determining concentration.  However the addition of a light scattering detector capable of both dynamic and static light scattering measurements gives significant benefits.  Absolute molecular weight determination with enhanced light scattering response ensures that dimers are clearly identified, through both absolute molecular weight measurement and the enhanced response afforded by the LS detector towards higher molecular weight species.  Incorporating dynamic light scattering allows hydrodynamic radius determination providing the possibility of detecting conformational differences.  Furthermore, with the advent of antibody-drug conjugates (ADCs) where non-specific interactions are more likely to cause retention time differences, the light scattering detector provides greater reassurance and confidence in analytical data.  Join the Agilent experts for this discussion on the AdvanceBio and Agilent Infinity II approach to this area of application.  An open Q&A session on size exclusion in general will follow the talk.

    For Research Use Only. Not for use in diagnostic procedures.

    Show Resources
    You May Also Like
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    APR 25, 2018 07:00 AM PDT
    C.E. CREDITS
    APR 25, 2018 07:00 AM PDT
    DATE: April 25, 2018TIME: 1500 CET, 10:00 a.m. EST, 7:00 a.m. PST Hematology and coagulation laboratories perform a wide range of routine and specialized tests, allowing clinicians to...
    SEP 12, 2017 08:00 AM PDT
    SEP 12, 2017 08:00 AM PDT
    DATE: September 12, 2017TIME: 8:00am PT, 11:00am ETEvery year, millions of dollars are wasted on poorly characterized and performing antibodies.  Key researchers in the antibody co...
    AUG 09, 2018 05:00 PM CEST
    C.E. CREDITS
    AUG 09, 2018 05:00 PM CEST
    DATE:  August 9, 2018TIME:  8:00am PDT, 11:00am EDT Current exon-level arrays and NGS techniques for CNV detection are plagued with a high number of false-positive...
    NOV 15, 2017 09:00 AM PST
    C.E. CREDITS
    NOV 15, 2017 09:00 AM PST
    DATE: November 15, 2017TIME: 09:00am PST, 12:00pm ESTMicroRNAs (miRNA) are a class of small non-coding RNAs (approximately 21 nt long) that bind complementary sequences in target mRNAs...
    Loading Comments...