AUG 17, 2016 8:00 AM PDT

A Comparison of the Challenges in the Quantification of Fosetyl-Al Tree Nut Residues with CESI-MS/MS and LC-MS/MS

Sponsored by: SCIEX, SCIEX
Speaker

Abstract

The export of tree nuts to the European Union (EU) is subject to a maximum residue limit (MRL) of 1.5 ppm for the fungicide fosetyl-Al. As fosetyl-Al readily hydrolyzes to phosphonic acid, the residue of fosetyl-Al is quantitated as the “sum of fosetyl, phosphonic acid, and their salts”. Unfortunately, phosphonate in tree nut extracts can originate from several sources other than fosetyl-Al, particularly when analyzed according to the EURL-SRM method, and if levels of fosetyl-Al exceed the MRL it impacts the export of tree nuts to the EU from the US (valued at $2.7 billion in 2014). The results from a multi-instrument, inter-laboratory study have recently demonstrated the limitations of the current EU methodology, which include:: poor extraction efficiency in tree nuts, low repeatability and of particular concern, false positive results obtained when high levels of phosphate (regularly found in California tree nuts) are present. In this presentation we will discuss the use of capillary electrophoresis, coupled to tandem mass spectrometry (CESI-MS/MS), an orthogonal technique to LC-MS/MS, which has been found to offer a promising alternative addressing these challenges.

Learning Objectives:

  • Learn about the challenges the tree nut industry is currently facing for exporting tree nuts into the EU
  • See the results produced from a multi-instrument, inter-laboratory study demonstrating the limitations of current methodology for the analysis of fungicides.
  • Learn about an alternative CESI-MS/MS based methodology for analyzing fungicides which overcomes the limitations and technical challenges present with currently utilized methods.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
MAR 25, 2021 8:00 AM PDT
C.E. CREDITS
MAR 25, 2021 8:00 AM PDT
Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
APR 20, 2021 11:00 AM PDT
C.E. CREDITS
APR 20, 2021 11:00 AM PDT
Date: April 20, 2021 Time: 11:00am (PST), 2:00pm (EST) Identification and confirmation of microbial isolates is crucial in the analysis workflow, providing decision-makers the information...
APR 20, 2021 7:00 AM PDT
APR 20, 2021 7:00 AM PDT
Date: April 20, 2021 Time: 7:00am (PDT), 10:00am (EST) There is a vital need to characterize Biopharmaceuticals in early phase screening to ensure the most suitable candidates are accelerate...
MAY 27, 2021 8:00 AM PDT
MAY 27, 2021 8:00 AM PDT
Date: May 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) With social distancing measures still enforced in large parts of the world, many researchers must minimize time in the lab where they may...
APR 19, 2021 8:00 AM PDT
C.E. CREDITS
APR 19, 2021 8:00 AM PDT
Date: Apri 19, 2021 Time: 8:00am PDT, 5:00pm CEST Since the inception of modern biological research, BioSafety has evolved into a critical consideration in Good Laboratory Practices (GLP). Wi...
Loading Comments...
Show Resources
Attendees
  • See more