AUG 11, 2016 11:00 AM PDT

RNA-ISH for Long non-coding RNAs - new game changers in pathology

Speaker
  • Assistant Professor of Pathology Member-Michigan Center for Translational Pathology MLabs Genitourinary Service Line Director Co-Director, University of Michigan Rapid Autopsy Discovery Progr
    BIOGRAPHY

Abstract
DATE: August 11, 2016
TIME: 11:00AM PST, 2:00PM ET, 7:00PM GMT



The central dogma that forms the backbone of molecular biology is that DNA codes for RNA (transcription) which then codes for proteins (translation). However, studies over the last few years demonstrated that just 1.5% of the 3 billion base pairs of DNA constituting the human genome generate the ~20,000 protein coding genes, whereas at least 90% of genome is transcribed into non-coding RNA (ncRNA) that does not result in a protein product.  The rapid characterization of numerous ncRNAs has dramatically altered our understanding of biology.  The discovery of long ncRNAs (lncRNAs, longer than 200 nucleotides) as mediators of various cellular processes as well as various human pathophysiologic processes including cancer has established their role as critical biological entities.  Several lncRNAs have been linked to oncogenesis; for example, HOTAIR in breast and colon cancer, and SChLAP1, PCAT-1 and ANRIL in prostate cancer. SChLAP1 in situ hybridization (RNA-ISH) has been recently demonstrated by our group to be a novel tissue-based biomarker assay for outcome prognostication after radical prostatectomy that carries great clinical promise to help direct risk stratification for patients with prostate cancer. Because RNA-ISH assay is evaluated using a light microscope, it not only allows detection of lncRNA of interest, it provides insight into the localization of the lncRNA as well. Overall, a myriad of lncRNAs are associated with carcinogenesis and RNA-ISH provides a practical tool for clinical application of lncRNA markers of interest in situ.

Learning Objectives
  1. Understand the clinical potential and utility of lncRNAs
  2. Become familiar with RNA-ISH as a clinical translational too

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
MAY 18, 2021 7:00 AM PDT
MAY 18, 2021 7:00 AM PDT
Date: May 18, 2021 Time: 7:00am PDT, 10:00am EDT Proteins encoded by mutant genes in cancers can be processed and presented on tumor cell surface by human leukocyte antigen (HLA) molecules,...
Loading Comments...
Show Resources