SEP 22, 2020 8:00 AM PDT

Workflows for Glycosylation and Sialic Acid Analysis of Biotherapeutic Glycoproteins

Speaker
  • Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab
    BIOGRAPHY

Abstract
N-Glycans on biotherapeutics can affect immunogenicity, pharmacokinetics and pharmacodynamics, making characterization of N-glycans essential in the development process. Here we present rapid N-glycan sample preparation and analysis workflows for biotherapeutics, including released N-glycan labeling with InstantPC and 2-AB for characterization by liquid chromatography (LC) with fluorescence (FLD) and mass spec (MS) detection. In addition, we will discuss approaches to identify different sialylated N-glycan species as well as quantitating the sialic acid content of biotherapeutic glycoproteins using plate-based and LC/FLD/MS approaches.

Show Resources
You May Also Like
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
DEC 01, 2021 7:00 AM PST
C.E. CREDITS
DEC 01, 2021 7:00 AM PST
Date: December 01, 2021 Time: 7:00am (PST), 10:00am (EST) In the era of immuno-oncology, there is a growing need for the identification of new biomarkers predictive for sensitivity to anti-P...
SEP 16, 2021 8:00 AM PDT
C.E. CREDITS
SEP 16, 2021 8:00 AM PDT
Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
DEC 07, 2021 10:00 AM PST
C.E. CREDITS
DEC 07, 2021 10:00 AM PST
Date: December 07, 2021 Time: 10:00am (PST) The RNAscope™ technology allows high sensitivity and spatial resolution providing pivotal single-cell information to gain better insights in...
C.E. CREDITS
In-house testing is not required federally; however, states differ in their requirements. Cannabis extraction laboratories need to test extracts for pesticides, heavy metals, residual solven...
Loading Comments...
Show Resources