SEP 22, 2020

Agilent Biopharma Workflow Solutions Virtual Symposium: Advances in Glycan and Sialic Acid Analysis

Agilent Technologies is pleased to bring you the Biopharma Workflow Symposium: Advances in Glycan and Sialic Acid Analysis. This one-day virtual event aims to serve as a platform to bring the biopharma community together to discuss the latest innovations and end-to-end workflow solutions in glycan and sialic acid analysis. Agilent offers a complete portfolio of samples to answers solutions to allow you to characterize released N-glycans, glycopeptides and intact glycoproteins efficiently and with confidence.

Join the Agilent Virtual event to:

Attend live and on-demand educational webinars to learn how to optimize and streamline your analysis.
Participate in a live panel discussion to stay connected to new developments in glycan analysis, further your research, and have your research bottleneck questions answered in an interactive format.
Access and download a variety of application notes, posters, white papers and brochures to enhance your knowledge.
Interact and network live with Agilent’s technical product experts.
Visit our virtual product booth to learn more about featured products, view videos, request literature, access resources and chat with sales and technical representatives.

At Agilent, we have an unwavering commitment to provide trusted answers to the challenges you face – through interacting and partnering with the global scientific community, we strive to accelerate your goals by bringing you solutions and insights that lead to greater outcomes.

Our virtual conference allows you to participate in a global setting with no travel or cost to you. The event will remain open 12 months from the date of the live event. The webinars will be available for unlimited on-demand viewing. We look forward to having your join us for the informative and interactive event.

Attend our live symposium – and get a limited-edition Agilent "I glyCAN" beaker mug by filling out a short survey. You must be registered to attend live event.

Speakers

Christopher Colangelo, PhD, MBA

Biopharma Business Development Leader
BIOGRAPHY
Kenda Evans, PhD

Field Application Scientist, Agilent Technologies
BIOGRAPHY
Rebecca Glaskin

Application Scientist, Agilent Technologies
BIOGRAPHY
Aled Jones

Marketing Manager, Biosconsumables - Agilent Technologies
BIOGRAPHY
Lance Kasper

Technical Specialist, Agilent Technologies
BIOGRAPHY
David Wong, PhD

Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies
BIOGRAPHY
John Yan, PhD

Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab
BIOGRAPHY

Show Resources

You May Also Like

MAR 23, 2022 6:00 AM PDT

Cannabis Sciences 2022

LabRoots is pleased to announce the 5th Annual 2021 Cannabis Sciences Virtual Event . LabRoots will host the event on March 23, 2022. The Cannabis Sciences planning committee will be plannin...

MAY 18, 2022 6:00 AM PDT

Laboratory Automation 2022

Welcome to the 6th Annual Laboratory Automation Virtual Event ; a free virtual conference for professionals interested in the most recent technologies for today’s labs. Lab automation...

NOV 10, 2021 6:00 AM PST

C.E. CREDITS

NOV 10, 2021 6:00 AM PST

Clinical Diagnostics & Research 2021

Twelve years ago Labroots launched a new system of learning for a global scientific audience. Now, the 2021 Clinical Diagnostics and Research Virtual Event...

NOV 09, 2022 6:00 AM PST

Clinical Diagnostics & Research 2022

Thirteen years ago Labroots launched a new system of learning for a global scientific audience. Now, the 2022 Clinical Diagnostics and Research Virtual Event will again bring together clinic...

DEC 07, 2021 12:30 PM EST

NCATS at 10: Improving Health for All Through Translational Science

Date: December 7, 2021 Time: 12:30-5:00 p.m. ET #NCATS10...

SEP 06, 2022 6:00 AM PDT

Microbiology Virtual Week 2022

LabRoots is excited to bring academia and industry, research experts, virologists, microbiologists, healthcare professionals, and leading biomedical scientists under one roof at our 8th Annu...

Agenda

Download Agenda

ondemand

SEP 22, 2020 2:15 PM PDT

Open Panel Discussion

John Yan, PhD, David Wong, PhD, Aled Jones

Watch Webinar

John Yan, PhD
Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab
BIOGRAPHY

David Wong, PhD
Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies
BIOGRAPHY

Aled Jones
Marketing Manager, Biosconsumables - Agilent Technologies
BIOGRAPHY
SEP 22, 2020 1:00 PM PDT

Analyzing mAb Glycosylation at Multiple Levels Using the 6545XT AdvanceBio LC/Q-TOF

David Wong, PhD

Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies
BIOGRAPHY

Monoclonal antibodies (mAbs) and their derivatives represent a very important class of biopharmaceutical molecules with a wide range of applications. With the dramatic increase in approved mAb products and mAb product sales over the recent years, there is increased need for comprehensive analytical characterization capabilities. mAbs are heterogeneous molecules by nature which are composed of various types of sequences, modifications and structural variants. Protein glycosylation is one of the major post-translational modifications (PTMs) of a mAb that plays an important role in many biological processes. The distribution and composition of the glycans bound to the mAb molecules can have an effect on therapeutic efficacy and immunogenicity, consistent glycosylation-associated quality control of therapeutic monoclonal antibodies (mAbs) has become a high priority in pharmaceutical bioprocessing.

There are typically four levels of LC/MS workflows for Glycan/Glycoforms in characterization: level 1 and level 2 focus on the analysis of glycoforms on the intact and reduced mAb molecules. Level 3 is the analysis of glycopeptide generated from proteolytical digestion of mAbs, commonly part of the peptide sequence mapping workflow. Level 4 is the characterization of glycans that have been released by enzymatic cleavage or other mechanism.

We present here the complete N-glycan quantitation workflow solutions using liquid chromatography (LC) with fluorescence detection (FLD) or mass spectrometry (MS) for four levels of biotherapeutic analysis: intact mass, mAb subunits, glycopeptides, and released glycan. These solutions dramatically improve not only productivity by allowing convenient sample preparation and streamlined data acquisition, but also accuracy in data analysis.

Watch Webinar
SEP 22, 2020 11:45 AM PDT

Automated Sample Preparation for Biopharmaceutical Analysis: Removing Inefficiencies and Variability

Kenda Evans, PhD

Field Application Scientist, Agilent Technologies
BIOGRAPHY

Watch Webinar
SEP 22, 2020 10:30 AM PDT

Agilent LC-MS Released Glycan Workflow

Christopher Colangelo, PhD, MBA

Biopharma Business Development Leader
BIOGRAPHY

The structure of N-linked glycans can play a critical role in the pharmacology of therapeutic proteins, potentially affecting immunogenicity, pharmacokinetics and pharmacodynamics. This makes the characterization of N-glycans an essential part of the biotherapeutic development process. Released glycan mapping is the preferred method used to identify glycosylation sites and total glycan composition. We present Agilent’s comprehensive workflow for N-linked glycan utilizing automated N-linked Glycan sample preparation and high-resolution LC-MS data analysis.

Watch Webinar
Characterization of N-linked glycans with the Released Glycans Workflow of MassHunter Bioconfirm Software

Rebecca Glaskin

Application Scientist, Agilent Technologies
BIOGRAPHY

Monoclonal antibodies are heterogeneous molecules that include glycosylation variants, carboxyl truncation, and deamidation that require extensive analytical characterization. These molecules are one of the fastest growing classes in the biopharmaceutical industry. This presentation will discuss a complete workflow for intact monoclonal antibody analysis with the use of the 6545XT AdvanceBio LC/Q-TOF platform and automatic data processing with MassHunter BioConfirm software.

Watch Webinar
SEP 22, 2020 9:15 AM PDT

Optimizing Glycan Separations and Analysis by HPLC

Lance Kasper

Technical Specialist, Agilent Technologies
BIOGRAPHY

This talk will explore HILIC chromatography methods for the analysis of released N-glycans labeled with InstantPC or 2-AB dyes. Best practices for robust methods will be introduced. Method development strategies for resolving critical pairs will be discussed including gradient and mobile phase optimization.

Watch Webinar
SEP 22, 2020 8:00 AM PDT

Workflows for Glycosylation and Sialic Acid Analysis of Biotherapeutic Glycoproteins

John Yan, PhD

Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab
BIOGRAPHY

N-Glycans on biotherapeutics can affect immunogenicity, pharmacokinetics and pharmacodynamics, making characterization of N-glycans essential in the development process. Here we present rapid N-glycan sample preparation and analysis workflows for biotherapeutics, including released N-glycan labeling with InstantPC and 2-AB for characterization by liquid chromatography (LC) with fluorescence (FLD) and mass spec (MS) detection. In addition, we will discuss approaches to identify different sialylated N-glycan species as well as quantitating the sialic acid content of biotherapeutic glycoproteins using plate-based and LC/FLD/MS approaches.

Watch Webinar

Speakers

Christopher Colangelo, PhD, MBA

Biopharma Business Development Leader

BIOGRAPHY
Kenda Evans, PhD

Field Application Scientist, Agilent Technologies

BIOGRAPHY
Rebecca Glaskin

Application Scientist, Agilent Technologies

BIOGRAPHY
Aled Jones

Marketing Manager, Biosconsumables - Agilent Technologies

BIOGRAPHY
Lance Kasper

Technical Specialist, Agilent Technologies

BIOGRAPHY
David Wong, PhD

Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies

BIOGRAPHY
John Yan, PhD

Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab

BIOGRAPHY

Help

General Labroots Questions

email support@labroots.com

Virtual Events and Webinars

Supported Configurations

Operating System	Internet Explorer	Firefox	Chrome	Safari
Vista	IE8+	FF10+	Chrome15+
Windows 7	IE8+	FF10+	Chrome15+
Windows 8	IE10	FF10+	Chrome15+
Mac OS X		FF10+	Chrome15+	Safari5.1+

Cookie settings: session cookies enabled
Browser configuration must have JavaScript enabled
Adobe Flash player 10.2 or later

To watch our webinars and virtual events a modern browser such as Chrome, Firefox, Safari, or an updated version of Internet Explorer is required.

Internet Connection

A minimum of 100Kbps is recommended for audio-only events
A minimum of 300Kbps is recommended for speaker on-video events

Computer Equipment

Screen Resolution: Minimum 1024x768
Video Card: 65,000 colors minimum
Sound card and speakers (or headphones) for hearing webcast in full format
Audio Card: 16-bit minimum
RAM: 512MB of free RAM

Firewall Protocol

HTTP on port 80 or HTTPS on port 443
Adobe RTMP on port 1935, 80, or 443 with automatic fall-back to RTMPT (RTMP tunneling) on ports 1935, 80 or 443
RTMPE (RTMP encrypted) on ports 1935, 80 or 443

Work-At-Home Attendees

If a corporate VPN is used, confirm that your network traffic is not directed over the company VPN. If it is, please turn off the VPN while attending the virtual event.

For A Seamless Experience

Registration is required for most events
Adobe Flash Player is needed to view webinar videos
Self Test your system compatibility ahead of time

When using a mobile device, FREE Mobile Apps are available:

- For viewing Webinars:

iPhone/iPad, download the Webcast Studio App
Android device, download the Webcast Studio App

- For viewing Virtual Events

iPhone/iPad, download the Virtual Environments App
Android, download the Virtual Environments App

Attendee Guide

To learn more about how the virtual event works for attendees, download the Attendee Guide (PDF).

Virtual Event and Webinar Support:

Email: support@labroots.com
Webpage: About Virtual Events

Continuing Education (CME/CE) Support

Phone: +1-714-930-3518
Email: CE@labroots.com
Webpage: Continuing Education CME/CE Credits

Agilent Biopharma Workflow Solutions Virtual Symposium: Advances in Glycan and Sialic Acid Analysis

Christopher Colangelo, PhD, MBA

Kenda Evans, PhD

Rebecca Glaskin

Aled Jones

Lance Kasper

David Wong, PhD

John Yan, PhD