SEP 22, 2020

Agilent Biopharma Workflow Solutions Virtual Symposium: Advances in Glycan and Sialic Acid Analysis

Agilent Technologies is pleased to bring you the Biopharma Workflow Symposium: Advances in Glycan and Sialic Acid Analysis. This one-day virtual event aims to serve as a platform to bring the biopharma community together to discuss the latest innovations and end-to-end workflow solutions in glycan and sialic acid analysis. Agilent offers a complete portfolio of samples to answers solutions to allow you to characterize released N-glycans, glycopeptides and intact glycoproteins efficiently and with confidence.

Join the Agilent Virtual event to:

Attend live and on-demand educational webinars to learn how to optimize and streamline your analysis.
Participate in a live panel discussion to stay connected to new developments in glycan analysis, further your research, and have your research bottleneck questions answered in an interactive format.
Access and download a variety of application notes, posters, white papers and brochures to enhance your knowledge.
Interact and network live with Agilent’s technical product experts.
Visit our virtual product booth to learn more about featured products, view videos, request literature, access resources and chat with sales and technical representatives.

At Agilent, we have an unwavering commitment to provide trusted answers to the challenges you face – through interacting and partnering with the global scientific community, we strive to accelerate your goals by bringing you solutions and insights that lead to greater outcomes.

Our virtual conference allows you to participate in a global setting with no travel or cost to you. The event will remain open 12 months from the date of the live event. The webinars will be available for unlimited on-demand viewing. We look forward to having your join us for the informative and interactive event.

Attend our live symposium – and get a limited-edition Agilent "I glyCAN" beaker mug by filling out a short survey. You must be registered to attend live event.

Speakers

Christopher Colangelo, PhD, MBA

Biopharma Business Development Leader
Kenda Evans, PhD

Field Application Scientist, Agilent Technologies
Rebecca Glaskin

Application Scientist, Agilent Technologies
Aled Jones

Marketing Manager, Biosconsumables - Agilent Technologies
Lance Kasper

Technical Specialist, Agilent Technologies
David Wong, PhD

Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies
John Yan, PhD

Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab

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SEP 22, 2020 1:00 PM PDT

Analyzing mAb Glycosylation at Multiple Levels Using the 6545XT AdvanceBio LC/Q-TOF

David Wong, PhD

Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies

Monoclonal antibodies (mAbs) and their derivatives represent a very important class of biopharmaceutical molecules with a wide range of applications. With the dramatic increase in approved mAb products and mAb product sales over the recent years, there is increased need for comprehensive analytical characterization capabilities. mAbs are heterogeneous molecules by nature which are composed of various types of sequences, modifications and structural variants. Protein glycosylation is one of the major post-translational modifications (PTMs) of a mAb that plays an important role in many biological processes. The distribution and composition of the glycans bound to the mAb molecules can have an effect on therapeutic efficacy and immunogenicity, consistent glycosylation-associated quality control of therapeutic monoclonal antibodies (mAbs) has become a high priority in pharmaceutical bioprocessing.

There are typically four levels of LC/MS workflows for Glycan/Glycoforms in characterization: level 1 and level 2 focus on the analysis of glycoforms on the intact and reduced mAb molecules. Level 3 is the analysis of glycopeptide generated from proteolytical digestion of mAbs, commonly part of the peptide sequence mapping workflow. Level 4 is the characterization of glycans that have been released by enzymatic cleavage or other mechanism.

We present here the complete N-glycan quantitation workflow solutions using liquid chromatography (LC) with fluorescence detection (FLD) or mass spectrometry (MS) for four levels of biotherapeutic analysis: intact mass, mAb subunits, glycopeptides, and released glycan. These solutions dramatically improve not only productivity by allowing convenient sample preparation and streamlined data acquisition, but also accuracy in data analysis.

SEE ABSTRACT HIDE ABSTRACT
SEP 22, 2020 11:45 AM PDT

Automated Sample Preparation for Biopharmaceutical Analysis: Removing Inefficiencies and Variability

Kenda Evans, PhD

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SEP 22, 2020 10:30 AM PDT

Agilent LC-MS Released Glycan Workflow

Christopher Colangelo, PhD, MBA

Biopharma Business Development Leader

The structure of N-linked glycans can play a critical role in the pharmacology of therapeutic proteins, potentially affecting immunogenicity, pharmacokinetics and pharmacodynamics. This makes the characterization of N-glycans an essential part of the biotherapeutic development process. Released glycan mapping is the preferred method used to identify glycosylation sites and total glycan composition. We present Agilent’s comprehensive workflow for N-linked glycan utilizing automated N-linked Glycan sample preparation and high-resolution LC-MS data analysis.

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Characterization of N-linked glycans with the Released Glycans Workflow of MassHunter Bioconfirm Software

Rebecca Glaskin

Application Scientist, Agilent Technologies

Monoclonal antibodies are heterogeneous molecules that include glycosylation variants, carboxyl truncation, and deamidation that require extensive analytical characterization. These molecules are one of the fastest growing classes in the biopharmaceutical industry. This presentation will discuss a complete workflow for intact monoclonal antibody analysis with the use of the 6545XT AdvanceBio LC/Q-TOF platform and automatic data processing with MassHunter BioConfirm software.

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SEP 22, 2020 9:15 AM PDT

Optimizing Glycan Separations and Analysis by HPLC

Lance Kasper

Technical Specialist, Agilent Technologies

This talk will explore HILIC chromatography methods for the analysis of released N-glycans labeled with InstantPC or 2-AB dyes. Best practices for robust methods will be introduced. Method development strategies for resolving critical pairs will be discussed including gradient and mobile phase optimization.

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SEP 22, 2020 8:00 AM PDT

Workflows for Glycosylation and Sialic Acid Analysis of Biotherapeutic Glycoproteins

John Yan, PhD

Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab

N-Glycans on biotherapeutics can affect immunogenicity, pharmacokinetics and pharmacodynamics, making characterization of N-glycans essential in the development process. Here we present rapid N-glycan sample preparation and analysis workflows for biotherapeutics, including released N-glycan labeling with InstantPC and 2-AB for characterization by liquid chromatography (LC) with fluorescence (FLD) and mass spec (MS) detection. In addition, we will discuss approaches to identify different sialylated N-glycan species as well as quantitating the sialic acid content of biotherapeutic glycoproteins using plate-based and LC/FLD/MS approaches.

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Speakers

Christopher Colangelo, PhD, MBA
Biopharma Business Development Leader

Biography
Christopher Colangelo, PhD, MBA is the Agilent Biopharma Business Development Leader for the US and Canada. Prior to joining Agilent, he was the 5th employee and co-inventor of the 454-sequencing platform. In 2003, Chris joined Yale University as a Research Faculty in where he mentored and supervised a research group producing 60+ peer-reviewed publications and directed core research laboratories for four NIH P30 grants, totaling over $21 million worth of research funds.
Kenda Evans, PhD
Field Application Scientist, Agilent Technologies

Biography
Dr. Evans received her Ph.D. in Pharmacology from The University of Texas Health Science Center at San Antonio focusing on Serotonin 1A receptor pharmacology. She did a post-doc at the University of Houston, College of Pharmacy and Pharmaceutical Sciences concentrating on cardiovascular and respiratory pharmacology. Kenda joined Encysive Pharmaceuticals in the high throughput screening lab where she worked through many screening campaigns and had specific project management responsibilities. After 5 years with Encysive Pharmaceuticals, she joined PerkinElmer as a field application scientist with the high throughput screening reagents and plate readers group. In 2011 Kenda joined Agilent Technologies as a product specialist in the automation workflow group where she continues to work to build out new applications using the Bravo liquid handler for automated sample preparation. The combination of formal training in Pharmacology and the more than 9 years of experience in the automation division at Agilent allow Kenda to enable many efficient workflow solutions for all automation needs.
Rebecca Glaskin
Application Scientist, Agilent Technologies

Biography
Rebecca Glaskin is an LC/MS Application Scientist at Agilent with a focus on BioPharma applications supporting the LC-Q/TOF and IM-QTOF platforms. Prior to joining Agilent, Rebecca received her Ph.D. in analytical chemistry from Indiana University in the lab of Professor David Clemmer. While there she designed and constructed home-built instruments, pushing the limits of the mobility resolution that can be obtained with a circular drift tube for the separation of biomolecules (peptides, proteins, carbohydrates, and metabolites). While there, she also studied hydrogen/deuterium exchange of proteins in the gas-phase as a function of time and pressure. Rebecca then went to Boston University as a Postdoctoral Associate in the lab of Professor Catherine Costello to develop a database containing collision cross section values for glycans, peptides, and glycopeptides utilizing Agilent Technologies 6560 IM-QTOF. This database can be used to determine how the collision cross section is altered with the addition of individual saccharide units. The trendlines obtained from this database will be used to predict collision cross sections for glycopeptides based on the conformation and structure of the specific glycoform.
Aled Jones
Marketing Manager, Biosconsumables - Agilent Technologies

Biography
Aled Jones is Marketing Manager for Biosconsumables at Agilent, focusing on tools for glycobiology, and joined the company via the acquisition of Bay Area-based ProZyme by Agilent Technologies. He received his B.Sc. and Ph.D. degrees in biochemistry from Cardiff University, completing graduate work in the connective tissue biology laboratories. This was followed by a postdoctoral fellowship at Wyeth Research in Cambridge MA, where he subsequently worked as a Senior Research Scientist.
Lance Kasper
Technical Specialist, Agilent Technologies

Biography
Lance Kasper has a Masters in Medical Biotechnology from University of Illinois. His previous experience includes working at Eprogen, a small CRO and developer of HPLC phases for biomolecule analysis. At Eprogen he performed 2D HPLC separations of complex protein samples using reversed phase and ion exchange methods. Prior to joining Agilent, Lance worked as an application specialist with Intavis Inc. At Intavis he supported lab automation platforms for peptide synthesis, in situ hybridization, and protein digestion for peptide mapping. Lance joined Agilent in 2016 as a technical specialist to support the growing HPLC columns portfolio for biomolecule analysis. At Agilent Lance assists customers with method development, column recommendations, and troubleshooting challenging biomolecule separations.
David Wong, PhD
Sr. Applications Scientist - LC/MS Solution, MSD Division, Agilent Technologies

Biography
Dr. Wong has more than 20 years of experience working in the biotech/pharmaceutical industry. Prior to joining Agilent, he worked as Research Scientist and the Proteomics (Mass Spectrometry) Group Leader at DuPont Industrial Bioscience Division (formally Genencor International, Inc.) located in Silicon Valley. Dr. Wong has strong expertise in both small and macromolecules characterization (Identification, Quantitation, Proteomics, Metabolomics and various BioPharma applications).

Dr. Wong received his Ph.D. in Biochemistry from University of California, Santa Barbara. He also served as a Post-doctoral Researcher at the UCSF Mass Spectrometry Facility, Dept. of Pharmaceutical Chemistry at University of California San Francisco.
John Yan, PhD
Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab

Biography
Dr. John Yan is an Applications Chemist for the Bioconsumables portfolio within the Chemistries and Supplies Division of Agilent CrossLab group. John joined Agilent as part of the ProZyme acquisition where he was an Applications Scientist supporting glycobiology reagents and N-glycan sample preparation and analytical workflows.

John has a background in biochemistry and analytical chemistry with a bachelor’s degree in chemistry from Oregon State University and a Ph.D. in chemistry from Portland State University. His graduate work was in the area of microbial natural products biosynthesis and genetic engineering. This was followed by postdoctoral work at Northwestern University focused on the utilization of mass spectrometry as a tool for natural products research and discovery. From there he joined Life Technologies (Thermo Fisher Scientific) as a Staff Scientist supporting the new product introduction and commercialization process.

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