Name: Caspase-3 assay Kit for drug discovery
Availability: InStock

Caspase-3 assay Kit for drug discovery | Creative BioMart

The CASPASE-3 Assay Kit for Drug Discovery is a complete assay system designed to screen caspase-3 inhibitors. It contains both a colorimetric substrate (DEVD-pNA) and a fluorogenic substrate (DEVD-AMC). Cleavage of the p-nitroanilide (pNA) from the colorimetric substrate increases absorption at 405nm. The fluorescent assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) dye from the C-terminus of the peptide substrate. Cleavage of the dye from the substrate increases its fluorescence intensity at 460 nm. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of caspase-3, a potential therapeutic target. An inhibitor, DEVD-CHO (aldehyde), is also included as a prototypic control inhibitor. The DEVD amino acid sequence is derived from the caspase-3 cleavage site in PARP.

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Creative BioMart is a professional biotechnology supplier focused on the development and production of recombinant protein products. Over the years, we have grown exponentially to offer over 100,000 protein products, including recombinant protein, native proteins, cell/tissue lysates, chromatography, lectins, and detection kits. In response to customers'...