mouse monoclonal IgG2b; AIF Antibody (E-1) is an IgG2b κ mouse monoclonal AIF antibody (also designated AIFM1 antibody) that detects the AIF protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. AIF Antibody (E-1) is available as both the non-conjugated anti-AIF antibody form, as well as multiple conjugated forms of anti-AIF antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.