mouse monoclonal IgG1; SLP-76 Antibody (F-7) is an IgG1 κ mouse monoclonal SLP-76 antibody (also designated LCP2 antibody, Lymphocyte Cytosolic Protein 2 antibody, SH2 Domain-Containing Leukocyte Protein Of 76 KDa antibody, 76 KDa Tyrosine Phosphoprotein antibody, or SLP-76 Tyrosine Phosphoprotein antibody) that detects the SLP-76 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. SLP-76 Antibody (F-7) is available as both the non-conjugated anti-SLP-76 antibody form, as well as multiple conjugated forms of anti-SLP-76 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav's function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.