mouse monoclonal IgG2a; granzyme B Antibody (2C5) is an IgG2a κ mouse monoclonal granzyme B antibody (also designated GRAB antibody, Granzyme B antibody, T-Cell Serine Protease 1-3E antibody, Cathepsin G-Like 1 antibody, CTSGL1 antibody, CGL1 antibody, SECT antibody, HLP antibody, Cytotoxic T-Lymphocyte Proteinase 2 antibody, Cytotoxic Serine Protease B antibody, Human Lymphocyte Protein antibody, Fragmentin 2 antibody, CTLA1 antibody, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1 antibody, Lymphocyte Protease antibody, Granzyme 2 antibody, CTLA-1 antibody, or GRB antibody) that detects the granzyme B protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. granzyme B Antibody (2C5) is available as both the non-conjugated anti-granzyme B antibody form, as well as multiple conjugated forms of anti-granzyme B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly (ADP ribose) polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.