mouse monoclonal IgG1; NFATc3 Antibody (F-1) is an IgG1 κ mouse monoclonal NFATc3 antibody (also designated NFAT4 antibody, or nuclear factor of activated T cells 3 antibody) that detects the NFATc3 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. NFATc3 Antibody (F-1) is available as both the non-conjugated anti-NFATc3 antibody form, as well as multiple conjugated forms of anti-NFATc3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFκB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFATc1 (NFATc), NFATc2 (NFATp) and NFATc3 (NFAT4, NFSTx) are predominantly expressed in immune cells. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus.