mouse monoclonal IgG1; eIF2Bβ Antibody (P-4) is an IgG1 κ mouse monoclonal eIF2B beta antibody (also designated eIF2Bβ antibody) that detects the eIF2B beta protein of mouse, rat and human origin by WB, IF and ELISA. eIF2Bβ Antibody (P-4) is available as both the non-conjugated anti-eIF2B beta antibody form, as well as multiple conjugated forms of anti-eIF2B beta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ, and eIF2Bε. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity is exhibited by the eIF2Bε subunit alone, but is greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bα subunit.