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    Oligo Synthesis

    Oligo Synthesis: Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not have this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks derived from protected 2'-deoxynucleosides (dA, dC, dG, and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, e.g. LNA or BNA.

    Trending News

    • MAY 18, 2021
      Genetics & Genomics
      Library Prep Methods for SARS-CoV-2 Sequencing: a Summary
      Library Prep Methods for SARS-CoV-2 Sequencing: a Summary
      As the SARS-CoV-2 virus continues to mutate, rapid sequencing of COVID-19-positive samples is more critical than ever. N ...
      Sponsored By: Roche Diagnostics
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      OCT 30, 2018
      Drug Discovery & Development
      Re-sensitizing Drug-resistant Human Tumor Cells
      Re-sensitizing Drug-resistant Human Tumor Cells
      Understanding how cancer cells avoid death despite their DNA being damaged will create new strategies to enhance cancer ...
      Written By: Nouran Amin
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    MAY 18, 2021
    Genetics & Genomics
    Library Prep Methods for SARS-CoV-2 Sequencing: a Summary
    Library Prep Methods for SARS-CoV-2 Sequencing: a Summary
    As the SARS-CoV-2 virus continues to mutate, rapid sequencing of COVID-19-positive samples is more critical than ever. N ...
    Sponsored By: Roche Diagnostics
    2
    OCT 30, 2018
    Drug Discovery & Development
    Re-sensitizing Drug-resistant Human Tumor Cells
    Re-sensitizing Drug-resistant Human Tumor Cells
    Understanding how cancer cells avoid death despite their DNA being damaged will create new strategies to enhance cancer ...
    Written By: Nouran Amin
    2
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