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    PCR method

    Polymerase Chain Reaction (PCR) is a laboratory method in which a specific segment of genetic material is amplified by undergoing multiple rounds of DNA synthesis. PCR utilizes synthetic fragments of DNA, made up of a specific sequence of nucleotides, designed to select the desired segment of DNA.

    Webinars

    • NOV 12, 2020 | 12:00 PM
      Measuring levels of neutralizing antibodies in SARS CoV-2 infected/recovered and vaccinated individuals using a high-throughput and cost-effective test
      Measuring levels of neutralizing antibodies in SARS CoV-2 infected/recovered...
      As many parts of the northern hemisphere move into the colder months and indoors, SARS CoV-2 has begun a second infection wave that has exceeded the first surge of cases in many global regio...
      Speaker: Sean Taylor, MBA, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2020
      Sponsored By: GenScript
      2
      OCT 22, 2020 | 8:00 AM
      Driving lab efficiency with PCR power
      Driving lab efficiency with PCR power
      Abstract: Detection and/or amplification of target DNA sequences by polymerase chain reaction (PCR) is a prevalent method in molecular biology. PCR has a broad range of applications, includi...
      Speaker: Kyle Hondorp , Marija Navickaitė
      Sponsored By: Thermo Fisher Scientific
      150 2
      OCT 13, 2020 | 5:00 AM
      Lifting the Veil on Innovations and Product Launches 2020
      Lifting the Veil on Innovations and Product Launches 2020
      Date: October 13, 2020 Time: 5:00am (PDT), 8:00am (EDT) Dr. Markus Kostrzewa shall ellaborate the progress that has been made in Bruker Microbiology Research and Development, including new p...
      Speaker: Markus Kostrzewa, PhD
      Sponsored By: Bruker Microbiology & Infection Diagnostics
      6 1
      SEP 17, 2020 | 4:30 PM
      Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
      Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
      The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PC...
      Speaker: Brian McNally, PhD
      Presented at: Coronavirus Series
      3
      SEP 17, 2020 | 3:30 PM
      SARS-CoV-2 Testing Today and a New Serology Approach For High Throughput Screening
      SARS-CoV-2 Testing Today and a New Serology Approach For High...
      Richard Hughes: New immunogenicity strategies to meet the needs of a developing pandemic Shortly after the COVID-19 pandemic began, the subject of serological testing for anti-viral antibodi...
      Speaker: Russell Garlick, PhD , Richard Hughes , Joel Lefferts, PhD, HCLD, DABCC
      Presented at: Coronavirus Series
      Sponsored By: LGC Seracare
      1
      SEP 10, 2020 | 11:00 AM
      Optimizing the extraction of human microbiome samples
      Optimizing the extraction of human microbiome samples
      Learning Objectives: 1. Understand the challenges of extracting DNA from human samples for microbiome analysis, and learn about the best technologies for accomplishing this 2. Learn about pr...
      Speaker: Dominic O'Neil, MS, MBA
      Presented at: Microbiology Week Virtual Event Series 2020
      Sponsored By: QIAGEN
      2
      SEP 04, 2020 | 8:00 AM
      Incorporation and Automation of RNAscope Technology into your COVID-19 Viral Pathogenesis Research
      Incorporation and Automation of RNAscope Technology into your...
      Date: September 4, 2020 Time: 8:00am (PTD), 11:00am (ETD) Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the...
      Speaker: Jyoti Phatak-Sheldon , Andrea De Biase, M.S. , Damian Cockfield
      Sponsored By: Leica Biosystems, ACD - A Bio-Techne Brand
      14 8
      SEP 03, 2020 | 9:00 AM
      The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
      The Complexities of SV Analytical Ultracentrifugation: AAVs are...
      DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
      Speaker: Dr. Lake Paul
      Sponsored By: Beckman Coulter Life Sciences
      12
      AUG 20, 2020 | 10:00 AM
      A Novel Method for the Isolation of Highly Pure Carcinoma Cell Populations from FFPE Tissue Improves Sensitivity for Detecting Somatic Mutations
      A Novel Method for the Isolation of Highly Pure Carcinoma Cell...
      DATE: August 20, 2020 TIME: 10:00am PT, 1:00pm ET Millions of formalin-fixed paraffin-embedded (FFPE) tissue specimens are archived annually. The enormous number of FFPE specimens available...
      Speaker: Carina Emery
      Sponsored By: Miltenyi Biotec
      21 1
      JUL 22, 2020 | 10:00 AM
      Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
      Molecular Diagnostic Solutions in a Pandemic: Planning for the...
      DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
      Speaker: Heba Mostafa, MD, PhD, D(ABMM)
      Sponsored By: GenMark Dx
      27 13
      JUL 02, 2020 | 1:30 PM
      Molecular Diagnosis of Genetic Diseases using CE in an era of different technologies
      Molecular Diagnosis of Genetic Diseases using CE in an era of...
      Genetic diseases are known occur due to various types of mutations in the human genome. The molecular assays to diagnose these diseases have been developed based on the type of mutation comm...
      Speaker: Ashwin Dalal, MD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      3
      JUL 02, 2020 | 12:45 PM
      Strategies to normalize miRNA expression in serum and plasma
      Strategies to normalize miRNA expression in serum and plasma
      MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level. They are known to play a critical role in multiple biologi...
      Speaker: Emily Zeringer , Harita Veereshlingam, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      2
      JUL 02, 2020 | 12:15 PM
      Microsatellite instability analysis for studying immunotherapy and Lynch syndrome-related cancers
      Microsatellite instability analysis for studying immunotherapy...
      Microsatellite instability (MSI) is a hypermutation phenotype that results from impaired DNA mismatch repair (MMR). The presence of high levels of MSI (MSI-H) is potentially predictive of Ly...
      Speaker: Charles Baudo, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUL 01, 2020 | 2:30 PM
      Reproductive genetic solution on ion torrent NGS
      Reproductive genetic solution on ion torrent NGS
      NGS technology has grown leaps and bounds in reproductive genetics field. Infertility is on the rise and with the mushrooming of so many IVF clinics in our country and increasing number of I...
      Speaker: Ritu Hari, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      2
      JUL 01, 2020 | 12:45 PM
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
      NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
      Speaker: Prof. Dr. Michael Hummel
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUL 01, 2020 | 12:15 PM
      Ion Torrent NGS-based simple and intuitive solutions for microbiome research
      Ion Torrent NGS-based simple and intuitive solutions for microbiome...
      Here I review the applications of Ion Torrent NGS technology in the field of microbiome research and some recent publications about it. With Ion Torrent 16S rRNA next generation sequencing,...
      Speaker: Keun-Joon Park, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      2
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 25, 2020 | 7:00 AM
      Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
      Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
      DATE: June 25, 2020 TIME: 7:00am PT Genotyping assays are essential in a variety of plant and animal applications including Marker Assisted Selection (MAS) and Quantitative Trait Locus (QTL)...
      Speaker: Jürgen Zimmermann , Bjoern Textor
      Sponsored By: Beckman Coulter Life Sciences
      16
      JUN 18, 2020 | 9:00 AM
      Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
      Unveiling the role of T cell dysfunction in the development and...
      DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
      Speaker: Felipe Galvez Cancino, Ph.D.
      Sponsored By: Thermo Fisher Scientific/Gibco
      18 2
      JUN 17, 2020 | 12:00 PM
      High-Throughput Assay for Monitoring COVID19 Antibodies and Their Isotypes
      High-Throughput Assay for Monitoring COVID19 Antibodies and Their...
      The 2020 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to rage. While PCR-based assays are used for viral diagnosis, high through-put, rapid s...
      Speaker: Susan Zolla-Pazner, PhD
      Presented at: Coronavirus Series
      Sponsored By: Luminex - A DiaSorin Company
      1
      JUN 17, 2020 | 7:30 AM
      Development of Luminex Based Serological Test for COVID-19 Diagnosis and Monitoring
      Development of Luminex Based Serological Test for COVID-19 Diagnosis...
      Coronavirus disease 2019 (Covid-19) is a global pandemic that has impacted the lives of the entire world’s population. Accurate and effective testing mechanisms to identify those with...
      Speaker: Imad Tarhoni, MD, PhD
      Presented at: Coronavirus Series
      Sponsored By: Luminex - A DiaSorin Company
      2
      JUN 17, 2020 | 7:30 AM
      Advanced solutions for viral detection and immune monitoring
      Advanced solutions for viral detection and immune monitoring
      Severe COVID-19 is characterized by dramatic immune system activation and inflammation. Several studies to modulate the immune response and limit immunopathology are ongoing. To better under...
      Speaker: Joel Guthridge, PhD , Petter Brodin, MD, PhD
      Presented at: Coronavirus Series
      Sponsored By: Standard BioTools
      1
      JUN 17, 2020 | 7:30 AM
      Effective RNA extraction for COVID-19 testing
      Effective RNA extraction for COVID-19 testing
      The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
      Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
      Presented at: Coronavirus Series
      Sponsored By: Cytiva
      1
      JUN 17, 2020 | 12:00 AM
      Setting up a Coronavirus (COVID-19) Clinical Diagnostic Testing Lab? LIMS for Automation and Data Management
      Setting up a Coronavirus (COVID-19) Clinical Diagnostic Testing...
      With over 6.7 million cases and over 213 countries and territories affected worldwide, the novel coronavirus (COVID-19) has challenged the world in several ways. As the global pandemic sprea...
      Speaker: Shonali Paul, MBA
      Presented at: Coronavirus Series
      2 1
    NOV 12, 2020 | 12:00 PM
    Measuring levels of neutralizing antibodies in SARS CoV-2 infected/recovered and vaccinated individuals using a high-throughput and cost-effective test
    Measuring levels of neutralizing antibodies in SARS CoV-2 infected/recovered...
    As many parts of the northern hemisphere move into the colder months and indoors, SARS CoV-2 has begun a second infection wave that has exceeded the first surge of cases in many global regio...
    Speaker: Sean Taylor, MBA, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2020
    Sponsored By: GenScript
    2
    OCT 22, 2020 | 8:00 AM
    Driving lab efficiency with PCR power
    Driving lab efficiency with PCR power
    Abstract: Detection and/or amplification of target DNA sequences by polymerase chain reaction (PCR) is a prevalent method in molecular biology. PCR has a broad range of applications, includi...
    Speaker: Kyle Hondorp , Marija Navickaitė
    Sponsored By: Thermo Fisher Scientific
    150 2
    OCT 13, 2020 | 5:00 AM
    Lifting the Veil on Innovations and Product Launches 2020
    Lifting the Veil on Innovations and Product Launches 2020
    Date: October 13, 2020 Time: 5:00am (PDT), 8:00am (EDT) Dr. Markus Kostrzewa shall ellaborate the progress that has been made in Bruker Microbiology Research and Development, including new p...
    Speaker: Markus Kostrzewa, PhD
    Sponsored By: Bruker Microbiology & Infection Diagnostics
    6 1
    SEP 17, 2020 | 4:30 PM
    Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
    Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
    The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PC...
    Speaker: Brian McNally, PhD
    Presented at: Coronavirus Series
    3
    SEP 17, 2020 | 3:30 PM
    SARS-CoV-2 Testing Today and a New Serology Approach For High Throughput Screening
    SARS-CoV-2 Testing Today and a New Serology Approach For High...
    Richard Hughes: New immunogenicity strategies to meet the needs of a developing pandemic Shortly after the COVID-19 pandemic began, the subject of serological testing for anti-viral antibodi...
    Speaker: Russell Garlick, PhD , Richard Hughes , Joel Lefferts, PhD, HCLD, DABCC
    Presented at: Coronavirus Series
    Sponsored By: LGC Seracare
    1
     
     
  • SEP 10, 2020 | 11:00 AM
    Optimizing the extraction of human microbiome samples
    Optimizing the extraction of human microbiome samples
    Learning Objectives: 1. Understand the challenges of extracting DNA from human samples for microbiome analysis, and learn about the best technologies for accomplishing this 2. Learn about pr...
    Speaker: Dominic O'Neil, MS, MBA
    Presented at: Microbiology Week Virtual Event Series 2020
    Sponsored By: QIAGEN
    2
    SEP 04, 2020 | 8:00 AM
    Incorporation and Automation of RNAscope Technology into your COVID-19 Viral Pathogenesis Research
    Incorporation and Automation of RNAscope Technology into your...
    Date: September 4, 2020 Time: 8:00am (PTD), 11:00am (ETD) Direct detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the...
    Speaker: Jyoti Phatak-Sheldon , Andrea De Biase, M.S. , Damian Cockfield
    Sponsored By: Leica Biosystems, ACD - A Bio-Techne Brand
    14 8
    SEP 03, 2020 | 9:00 AM
    The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
    The Complexities of SV Analytical Ultracentrifugation: AAVs are...
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    Speaker: Dr. Lake Paul
    Sponsored By: Beckman Coulter Life Sciences
    12
    AUG 20, 2020 | 10:00 AM
    A Novel Method for the Isolation of Highly Pure Carcinoma Cell Populations from FFPE Tissue Improves Sensitivity for Detecting Somatic Mutations
    A Novel Method for the Isolation of Highly Pure Carcinoma Cell...
    DATE: August 20, 2020 TIME: 10:00am PT, 1:00pm ET Millions of formalin-fixed paraffin-embedded (FFPE) tissue specimens are archived annually. The enormous number of FFPE specimens available...
    Speaker: Carina Emery
    Sponsored By: Miltenyi Biotec
    21 1
    JUL 22, 2020 | 10:00 AM
    Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
    Molecular Diagnostic Solutions in a Pandemic: Planning for the...
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    Speaker: Heba Mostafa, MD, PhD, D(ABMM)
    Sponsored By: GenMark Dx
    27 13
     
     
  • JUL 02, 2020 | 1:30 PM
    Molecular Diagnosis of Genetic Diseases using CE in an era of different technologies
    Molecular Diagnosis of Genetic Diseases using CE in an era of...
    Genetic diseases are known occur due to various types of mutations in the human genome. The molecular assays to diagnose these diseases have been developed based on the type of mutation comm...
    Speaker: Ashwin Dalal, MD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    3
    JUL 02, 2020 | 12:45 PM
    Strategies to normalize miRNA expression in serum and plasma
    Strategies to normalize miRNA expression in serum and plasma
    MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level. They are known to play a critical role in multiple biologi...
    Speaker: Emily Zeringer , Harita Veereshlingam, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    2
    JUL 02, 2020 | 12:15 PM
    Microsatellite instability analysis for studying immunotherapy and Lynch syndrome-related cancers
    Microsatellite instability analysis for studying immunotherapy...
    Microsatellite instability (MSI) is a hypermutation phenotype that results from impaired DNA mismatch repair (MMR). The presence of high levels of MSI (MSI-H) is potentially predictive of Ly...
    Speaker: Charles Baudo, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
    JUL 01, 2020 | 2:30 PM
    Reproductive genetic solution on ion torrent NGS
    Reproductive genetic solution on ion torrent NGS
    NGS technology has grown leaps and bounds in reproductive genetics field. Infertility is on the rise and with the mushrooming of so many IVF clinics in our country and increasing number of I...
    Speaker: Ritu Hari, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    2
    JUL 01, 2020 | 12:45 PM
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
    NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
    Speaker: Prof. Dr. Michael Hummel
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
     
     
  • JUL 01, 2020 | 12:15 PM
    Ion Torrent NGS-based simple and intuitive solutions for microbiome research
    Ion Torrent NGS-based simple and intuitive solutions for microbiome...
    Here I review the applications of Ion Torrent NGS technology in the field of microbiome research and some recent publications about it. With Ion Torrent 16S rRNA next generation sequencing,...
    Speaker: Keun-Joon Park, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    2
    JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
    JUN 25, 2020 | 7:00 AM
    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
    DATE: June 25, 2020 TIME: 7:00am PT Genotyping assays are essential in a variety of plant and animal applications including Marker Assisted Selection (MAS) and Quantitative Trait Locus (QTL)...
    Speaker: Jürgen Zimmermann , Bjoern Textor
    Sponsored By: Beckman Coulter Life Sciences
    16
    JUN 18, 2020 | 9:00 AM
    Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
    Unveiling the role of T cell dysfunction in the development and...
    DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
    Speaker: Felipe Galvez Cancino, Ph.D.
    Sponsored By: Thermo Fisher Scientific/Gibco
    18 2
    JUN 17, 2020 | 12:00 PM
    High-Throughput Assay for Monitoring COVID19 Antibodies and Their Isotypes
    High-Throughput Assay for Monitoring COVID19 Antibodies and Their...
    The 2020 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to rage. While PCR-based assays are used for viral diagnosis, high through-put, rapid s...
    Speaker: Susan Zolla-Pazner, PhD
    Presented at: Coronavirus Series
    Sponsored By: Luminex - A DiaSorin Company
    1
     
     
  • JUN 17, 2020 | 7:30 AM
    Development of Luminex Based Serological Test for COVID-19 Diagnosis and Monitoring
    Development of Luminex Based Serological Test for COVID-19 Diagnosis...
    Coronavirus disease 2019 (Covid-19) is a global pandemic that has impacted the lives of the entire world’s population. Accurate and effective testing mechanisms to identify those with...
    Speaker: Imad Tarhoni, MD, PhD
    Presented at: Coronavirus Series
    Sponsored By: Luminex - A DiaSorin Company
    2
    JUN 17, 2020 | 7:30 AM
    Advanced solutions for viral detection and immune monitoring
    Advanced solutions for viral detection and immune monitoring
    Severe COVID-19 is characterized by dramatic immune system activation and inflammation. Several studies to modulate the immune response and limit immunopathology are ongoing. To better under...
    Speaker: Joel Guthridge, PhD , Petter Brodin, MD, PhD
    Presented at: Coronavirus Series
    Sponsored By: Standard BioTools
    1
    JUN 17, 2020 | 7:30 AM
    Effective RNA extraction for COVID-19 testing
    Effective RNA extraction for COVID-19 testing
    The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
    Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
    Presented at: Coronavirus Series
    Sponsored By: Cytiva
    1
    JUN 17, 2020 | 12:00 AM
    Setting up a Coronavirus (COVID-19) Clinical Diagnostic Testing Lab? LIMS for Automation and Data Management
    Setting up a Coronavirus (COVID-19) Clinical Diagnostic Testing...
    With over 6.7 million cases and over 213 countries and territories affected worldwide, the novel coronavirus (COVID-19) has challenged the world in several ways. As the global pandemic sprea...
    Speaker: Shonali Paul, MBA
    Presented at: Coronavirus Series
    2 1
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