Webinars

NOV 19, 2020 8:00 AM GMT

Highly specific multiplexed RNA imaging in tissues with split-FISH

Sponsored by: Andor

Speakers

Kok Hao Chen

Senior Research Scientist, Genome Institute of Singapore
BIOGRAPHY
Alan Mullan, PhD

Product Specialist for Microscopy Cameras at Andor Technology.
BIOGRAPHY

Abstract

Date: November 19, 2020

Time: 8:00am (GMT), 9:00am (CET), 4:00pm (CST)

We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues.

Learning Objectives

Multiplexed FISH is an attractive tool for spatial transcriptomics.
Advantages of split-FISH design in lowering background and false positive noise
Example applications in profiling RNA localizations in mouse organs

Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Show Resources

You May Also Like

MAY 17, 2022 9:00 AM PDT

On the way towards personalized medicine: Gene therapy AAV characterization...

Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...

JUN 28, 2022 7:00 AM PDT

sCMOS Cameras For Light-Sheet Microscopy

Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...

Speaker: Prof. Ulrich Kubitscheck , Dr. Abhishek Kumar , Matthew Kose-Dunn , Phil Allen

Sponsored By: Teledyne Photometrics

AUG 24, 2022 7:00 AM PDT

Light Field Microscopy Camera Solutions

Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...

Speaker: Matthew Kose-Dunn , Phil Allen , Liang Gao , Shu Jia

Sponsored By: Teledyne Photometrics

OCT 11, 2022 8:00 AM PDT

C.E. CREDITS

OCT 11, 2022 8:00 AM PDT

Smarter sample preparation for single-cell sequencing

Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...

JUN 21, 2022 6:00 AM PDT

The future of predictive genomics in Israel

Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...

Speaker: Zisis Kozlakidis , David Hanna , Dr. Yehudit Cohen , Sowmiya Moorthie , Shai Carmi

Sponsored By: Thermo Fisher Scientific

SEP 29, 2022 8:00 AM PDT

A novel approach for high resolution full and empty AAV analysis...

Date: September 29, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Adeno-associated virus (AAV) is one of the most widely used delivery vehicles in gene therapy. To ensure the safety...