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    Transcription

    Transcription is the first step in gene expression in which genetic information is copied from a DNA to an RNA format. Transcription is controlled by proteins known as transcription factors that regulate the expression of genes.

    Webinars

    • On Demand
      C.E. CREDITS
      Epithelial-Mesenchymal Transition in the Crosshairs: Novel Treatments to Kill Drug Resistant, Metastatic Cells in Cancer
      Epithelial-Mesenchymal Transition in the Crosshairs: Novel Treatments...
      Across many cancers, mortality is associated with metastasis and acquired drug resistance, both of which often manifest in recurrent disease. Central to these phenomena is epithelial to mese...
      Speaker: Cai Roberts, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2024
      2
      NOV 01, 2023 | 7:30 AM
      Keynote Presentation: Microfluidics and CRISPR-Diagnostics/Therapy with Live Q&A
      Keynote Presentation: Microfluidics and CRISPR-Diagnostics/Therapy...
      Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
      Speaker: Xingyu Jiang, PhD
      Presented at: CRISPR Virtual Event Series 2023
      1
      On Demand
      Engineering CRISPR/Cas12 Components Toward Simple and Robust Next-Generation Diagnostics
      Engineering CRISPR/Cas12 Components Toward Simple and Robust Next-Generation...
      Type V and VI CRISPR/Cas systems have emerged as promising cutting-edge diagnostic tools for nucleic acid detection....
      Speaker: Piyush K. Jain, PhD
      Presented at: CRISPR Virtual Event Series 2023
      1
      On Demand
      Building Amplification-Free CRISPR/Cas Biosensors Through Autocatalysis Reaction Induced by Circular DNA Nanostructure
      Building Amplification-Free CRISPR/Cas Biosensors Through Autocatalysis...
      Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
      Speaker: Yi Li, PhD
      Presented at: CRISPR Virtual Event Series 2023
      1
      OCT 24, 2023 | 10:00 AM
      An epic advance in epigenomics: Automated CUT&RUN enables chromatin profiling for scaled research & drug discovery applications
      An epic advance in epigenomics: Automated CUT&RUN enables chromatin...
      Dynamic changes in chromatin drive gene expression programs during cellular development and contribute to pathological changes underlying disease. To date, efforts to characterize chromatin...
      Speaker: Andrea Johnstone, Ph.D. , Lori Euler
      Sponsored By: Beckman Coulter Life Sciences
      30
      OCT 05, 2023 | 12:00 PM
      Optimizing RNA Manufacturing for Rapid RNA Therapeutics Development with Live Q&A
      Optimizing RNA Manufacturing for Rapid RNA Therapeutics Development...
      The recent advancements in RNA therapeutics have brought to light the need for mRNA molecules that possess better stability and functionality in vivo....
      Speaker: Fengmei Pi, PhD
      Presented at: Cancer Research & Immuno-Oncology Week Virtual Event Series 2023
      Sponsored By: GenScript
      1
      AUG 10, 2023 | 8:00 AM
      Current Landscape and Future Directions of Multi-specific Antibodies
      Current Landscape and Future Directions of Multi-specific Antibodies
      Antibody therapeutics is a fast-growing market boosted by developments in bispecific antibodies and in newly emerging tetra-specific antibodies. With the ability to recruit a combination of...
      Speaker: Shebna Massey, PhD
      Sponsored By: Sino Biological
      14
      JUL 26, 2023 | 1:50 PM
      Characterization and biodistribution of REGENXBIO NAV® platform capsids: under-employed gene therapy vector AAV7
      Characterization and biodistribution of REGENXBIO NAV® platform...
      The use of adeno-associated viruses (AAV) as gene delivery vectors has vast potential for the treatment of many severe human diseases. A small group of intensively studied AAV capsids have b...
      Speaker: Dr. Samantha Yost
      Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
      4
      MAR 30, 2023 | 5:10 PM
      Molecular analysis in the diagnosis and prognosis of haematological malignancies
      Molecular analysis in the diagnosis and prognosis of haematological...
      This talk will highlight key changes to the various guidelines in myeloid and lymphoid testing, commenting on the current and future landscape of biomarkers. The speakers will then share the...
      Speaker: Dr. Geetha Menon, MD , Lihui Wang, PhD
      Presented at: OncomineWorld 2024: A Virtual NGS Education Meeting
      0
      MAR 22, 2023 | 9:00 AM
      Keynote Presentation: Linking Single-Cell Function to Multi-omic Analysis Using 'Lab on a Particle' Technology with Live Q&A
      Keynote Presentation: Linking Single-Cell Function to Multi-omic...
      We have developed 3D-shaped hydrogel microparticle platforms to capture cells, as well as isolate and label their secretions. These “lab on a particle” systems enable sorting cel...
      Speaker: Dino Di Carlo, Ph.D.
      Presented at: Laboratory Automation Virtual Event Series 2023
      6
      MAR 22, 2023 | 6:00 AM
      Leveraging LIMS to Facilitate Quality
      Leveraging LIMS to Facilitate Quality
      Regulated laboratories must comply with numerous quality requirements that are put in place by regulatory agencies to ensure that accurate data is being generated and reported. The vast majo...
      Speaker: Christine Paszko, PhD, MT (ASCP)
      Presented at: Laboratory Automation Virtual Event Series 2023
      4
      MAR 16, 2023 | 10:00 AM
      Webinar: Setting the Precedent for Artificial Intelligence and Full Laboratory Automation
      Webinar: Setting the Precedent for Artificial Intelligence and...
      Date: March 16, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) On Thursday March 16, COPAN Diagnostics is pleased to release presentations given at an event held during ASM Microbe 202...
      Speaker: Caitlin Murphy, Ph.D. , Kileen Shier, Ph.D., MBA, D(ABMM), MLS(ASCP)CM , Susan Sharp, Ph.D., DABMM, FAAM
      Sponsored By: COPAN Diagnostics
      15
      FEB 15, 2023 | 7:00 AM
      Scattering Microscopy: Label-Free Imaging Approaches
      Scattering Microscopy: Label-Free Imaging Approaches
      Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
      Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
      Sponsored By: Teledyne Photometrics
      28
      FEB 14, 2023 | 10:00 AM
      A spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum
      A spatially-resolved transcriptional atlas of the murine dorsal...
      Date: February 14, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) The dorsal pontine tegmentum (PnTg) is part of the Pons, the largest section of the brainstem. It is a complex region...
      Speaker: Stefano Nardone, PhD , Cory Brannigan
      Sponsored By: Vizgen
      6
      NOV 03, 2022 | 6:00 AM
      Targeting The Glyco-immune Checkpoints, Siglecs, to Enhance Anti-Viral Innate Immune Functions
      Targeting The Glyco-immune Checkpoints, Siglecs, to Enhance Anti-Viral...
      Date: October 13, 2022 Time: 10:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Most known immune checkpoints exert their effects upon protein-protein interactions, such as the PD1 checkpoint on CD...
      Speaker: Mohamed Abdel-Mohsen
      Sponsored By: GenScript
      16
      OCT 20, 2022 | 9:00 AM
      Immune Secrets of the Lymph Node Revealed by Spatial Genomics
      Immune Secrets of the Lymph Node Revealed by Spatial Genomics
      Date: October 13, 2022 Time: 11:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Single-cell atlases mapping immune cells provide hints on how immune cells mature, attenuate, and disseminate through...
      Speaker: Jason Reeves
      Sponsored By: NanoString Technologies
      10 1
      OCT 19, 2022 | 1:30 PM
      A Phase 1 Trial of Cyclosporine for Hospitalized Patients with COVID-19
      A Phase 1 Trial of Cyclosporine for Hospitalized Patients with...
      COVID-19 remains a global health emergency with limited treatment options, lagging vaccine rates, and inadequate healthcare resources in the face of an ongoing calamity. The disease is chara...
      Speaker: Julia Han Noll, MS
      Presented at: Coronavirus Series
      Sponsored By: NanoString Technologies
      6
      OCT 13, 2022 | 9:00 AM
      Smart EPU with Embedded CryoSPARCâ„¢ Live: integrated real-time data analysis for high-quality 3D protein structures
      Smart EPU with Embedded CryoSPARCâ„¢ Live: integrated real-time...
      First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
      Speaker: Julio Ortiz , Edward Pryor, PhD , Ali Punjani
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      10
      SEP 29, 2022 | 7:00 AM
      Multidimensional Approach to Decoding the Mysteries of Animal Development
      Multidimensional Approach to Decoding the Mysteries of Animal...
      Date: September 29, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Development and evolution are controlled, to a large degree, by regions of genomic DNA called enhancers that encode...
      Speaker: Justin Crocker
      Sponsored By: GenScript
      7
      SEP 20, 2022 | 9:00 AM
      Breaking through RT-qPCR boundaries in gene expression analysis
      Breaking through RT-qPCR boundaries in gene expression analysis
      Date: September 20, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription...
      Speaker: Jennifer Sandoval, M.S.
      Sponsored By: Thermo Fisher Scientific
      28
      SEP 07, 2022 | 12:00 PM
      A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting CRISPR-based Sample Depletion and Metagenomic Sequencing with Live Q&A
      A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting...
      The lack of preparedness for detecting the highly infectious SARS-CoV-2 pathogen, the pathogen responsible for the COVID-19 disease, has caused enormous harm to public health and the economy...
      Speaker: Jon Armstrong, MSc
      Presented at: Microbiology Week Virtual Event Series 2022
      Sponsored By: Jumpcode Genomics
      5
      AUG 16, 2022 | 11:00 AM
      How to Leverage Laser Capture Microdissection Technology for Advanced Oncology Research
      How to Leverage Laser Capture Microdissection Technology for Advanced...
      Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
      Speaker: Katherine Walter
      Sponsored By: Thermo Fisher Scientific
      12
      AUG 10, 2022 | 10:00 AM
      Avoiding hurdles in molecular assay development
      Avoiding hurdles in molecular assay development
      Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
      Speaker: Naeem Nathoo
      Sponsored By: Thermo Fisher Scientific
      16
      JUL 27, 2022 | 9:00 AM
      Accelerating strain development process for CDMO and CRO companies
      Accelerating strain development process for CDMO and CRO companies
      Date: July 27, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Manufacturing of proteins, mRNA or small molecules requires comprehensive strain engineering approaches to increase yield...
      Speaker: Eric Abbate
      Sponsored By: Inscripta
      8 1
    On Demand
    C.E. CREDITS
    Epithelial-Mesenchymal Transition in the Crosshairs: Novel Treatments to Kill Drug Resistant, Metastatic Cells in Cancer
    Epithelial-Mesenchymal Transition in the Crosshairs: Novel Treatments...
    Across many cancers, mortality is associated with metastasis and acquired drug resistance, both of which often manifest in recurrent disease. Central to these phenomena is epithelial to mese...
    Speaker: Cai Roberts, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2024
    2
    NOV 01, 2023 | 7:30 AM
    Keynote Presentation: Microfluidics and CRISPR-Diagnostics/Therapy with Live Q&A
    Keynote Presentation: Microfluidics and CRISPR-Diagnostics/Therapy...
    Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
    Speaker: Xingyu Jiang, PhD
    Presented at: CRISPR Virtual Event Series 2023
    1
    On Demand
    Engineering CRISPR/Cas12 Components Toward Simple and Robust Next-Generation Diagnostics
    Engineering CRISPR/Cas12 Components Toward Simple and Robust Next-Generation...
    Type V and VI CRISPR/Cas systems have emerged as promising cutting-edge diagnostic tools for nucleic acid detection....
    Speaker: Piyush K. Jain, PhD
    Presented at: CRISPR Virtual Event Series 2023
    1
    On Demand
    Building Amplification-Free CRISPR/Cas Biosensors Through Autocatalysis Reaction Induced by Circular DNA Nanostructure
    Building Amplification-Free CRISPR/Cas Biosensors Through Autocatalysis...
    Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
    Speaker: Yi Li, PhD
    Presented at: CRISPR Virtual Event Series 2023
    1
    OCT 24, 2023 | 10:00 AM
    An epic advance in epigenomics: Automated CUT&RUN enables chromatin profiling for scaled research & drug discovery applications
    An epic advance in epigenomics: Automated CUT&RUN enables chromatin...
    Dynamic changes in chromatin drive gene expression programs during cellular development and contribute to pathological changes underlying disease. To date, efforts to characterize chromatin...
    Speaker: Andrea Johnstone, Ph.D. , Lori Euler
    Sponsored By: Beckman Coulter Life Sciences
    30
     
     
  • OCT 05, 2023 | 12:00 PM
    Optimizing RNA Manufacturing for Rapid RNA Therapeutics Development with Live Q&A
    Optimizing RNA Manufacturing for Rapid RNA Therapeutics Development...
    The recent advancements in RNA therapeutics have brought to light the need for mRNA molecules that possess better stability and functionality in vivo....
    Speaker: Fengmei Pi, PhD
    Presented at: Cancer Research & Immuno-Oncology Week Virtual Event Series 2023
    Sponsored By: GenScript
    1
    AUG 10, 2023 | 8:00 AM
    Current Landscape and Future Directions of Multi-specific Antibodies
    Current Landscape and Future Directions of Multi-specific Antibodies
    Antibody therapeutics is a fast-growing market boosted by developments in bispecific antibodies and in newly emerging tetra-specific antibodies. With the ability to recruit a combination of...
    Speaker: Shebna Massey, PhD
    Sponsored By: Sino Biological
    14
    JUL 26, 2023 | 1:50 PM
    Characterization and biodistribution of REGENXBIO NAV® platform capsids: under-employed gene therapy vector AAV7
    Characterization and biodistribution of REGENXBIO NAV® platform...
    The use of adeno-associated viruses (AAV) as gene delivery vectors has vast potential for the treatment of many severe human diseases. A small group of intensively studied AAV capsids have b...
    Speaker: Dr. Samantha Yost
    Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
    4
    MAR 30, 2023 | 5:10 PM
    Molecular analysis in the diagnosis and prognosis of haematological malignancies
    Molecular analysis in the diagnosis and prognosis of haematological...
    This talk will highlight key changes to the various guidelines in myeloid and lymphoid testing, commenting on the current and future landscape of biomarkers. The speakers will then share the...
    Speaker: Dr. Geetha Menon, MD , Lihui Wang, PhD
    Presented at: OncomineWorld 2024: A Virtual NGS Education Meeting
    0
    MAR 22, 2023 | 9:00 AM
    Keynote Presentation: Linking Single-Cell Function to Multi-omic Analysis Using 'Lab on a Particle' Technology with Live Q&A
    Keynote Presentation: Linking Single-Cell Function to Multi-omic...
    We have developed 3D-shaped hydrogel microparticle platforms to capture cells, as well as isolate and label their secretions. These “lab on a particle” systems enable sorting cel...
    Speaker: Dino Di Carlo, Ph.D.
    Presented at: Laboratory Automation Virtual Event Series 2023
    6
     
     
  • MAR 22, 2023 | 6:00 AM
    Leveraging LIMS to Facilitate Quality
    Leveraging LIMS to Facilitate Quality
    Regulated laboratories must comply with numerous quality requirements that are put in place by regulatory agencies to ensure that accurate data is being generated and reported. The vast majo...
    Speaker: Christine Paszko, PhD, MT (ASCP)
    Presented at: Laboratory Automation Virtual Event Series 2023
    4
    MAR 16, 2023 | 10:00 AM
    Webinar: Setting the Precedent for Artificial Intelligence and Full Laboratory Automation
    Webinar: Setting the Precedent for Artificial Intelligence and...
    Date: March 16, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) On Thursday March 16, COPAN Diagnostics is pleased to release presentations given at an event held during ASM Microbe 202...
    Speaker: Caitlin Murphy, Ph.D. , Kileen Shier, Ph.D., MBA, D(ABMM), MLS(ASCP)CM , Susan Sharp, Ph.D., DABMM, FAAM
    Sponsored By: COPAN Diagnostics
    15
    FEB 15, 2023 | 7:00 AM
    Scattering Microscopy: Label-Free Imaging Approaches
    Scattering Microscopy: Label-Free Imaging Approaches
    Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
    Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
    Sponsored By: Teledyne Photometrics
    28
    FEB 14, 2023 | 10:00 AM
    A spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum
    A spatially-resolved transcriptional atlas of the murine dorsal...
    Date: February 14, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) The dorsal pontine tegmentum (PnTg) is part of the Pons, the largest section of the brainstem. It is a complex region...
    Speaker: Stefano Nardone, PhD , Cory Brannigan
    Sponsored By: Vizgen
    6
    NOV 03, 2022 | 6:00 AM
    Targeting The Glyco-immune Checkpoints, Siglecs, to Enhance Anti-Viral Innate Immune Functions
    Targeting The Glyco-immune Checkpoints, Siglecs, to Enhance Anti-Viral...
    Date: October 13, 2022 Time: 10:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Most known immune checkpoints exert their effects upon protein-protein interactions, such as the PD1 checkpoint on CD...
    Speaker: Mohamed Abdel-Mohsen
    Sponsored By: GenScript
    16
     
     
  • OCT 20, 2022 | 9:00 AM
    Immune Secrets of the Lymph Node Revealed by Spatial Genomics
    Immune Secrets of the Lymph Node Revealed by Spatial Genomics
    Date: October 13, 2022 Time: 11:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Single-cell atlases mapping immune cells provide hints on how immune cells mature, attenuate, and disseminate through...
    Speaker: Jason Reeves
    Sponsored By: NanoString Technologies
    10 1
    OCT 19, 2022 | 1:30 PM
    A Phase 1 Trial of Cyclosporine for Hospitalized Patients with COVID-19
    A Phase 1 Trial of Cyclosporine for Hospitalized Patients with...
    COVID-19 remains a global health emergency with limited treatment options, lagging vaccine rates, and inadequate healthcare resources in the face of an ongoing calamity. The disease is chara...
    Speaker: Julia Han Noll, MS
    Presented at: Coronavirus Series
    Sponsored By: NanoString Technologies
    6
    OCT 13, 2022 | 9:00 AM
    Smart EPU with Embedded CryoSPARCâ„¢ Live: integrated real-time data analysis for high-quality 3D protein structures
    Smart EPU with Embedded CryoSPARCâ„¢ Live: integrated real-time...
    First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
    Speaker: Julio Ortiz , Edward Pryor, PhD , Ali Punjani
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    10
    SEP 29, 2022 | 7:00 AM
    Multidimensional Approach to Decoding the Mysteries of Animal Development
    Multidimensional Approach to Decoding the Mysteries of Animal...
    Date: September 29, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Development and evolution are controlled, to a large degree, by regions of genomic DNA called enhancers that encode...
    Speaker: Justin Crocker
    Sponsored By: GenScript
    7
    SEP 20, 2022 | 9:00 AM
    Breaking through RT-qPCR boundaries in gene expression analysis
    Breaking through RT-qPCR boundaries in gene expression analysis
    Date: September 20, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription...
    Speaker: Jennifer Sandoval, M.S.
    Sponsored By: Thermo Fisher Scientific
    28
     
     
  • SEP 07, 2022 | 12:00 PM
    A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting CRISPR-based Sample Depletion and Metagenomic Sequencing with Live Q&A
    A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting...
    The lack of preparedness for detecting the highly infectious SARS-CoV-2 pathogen, the pathogen responsible for the COVID-19 disease, has caused enormous harm to public health and the economy...
    Speaker: Jon Armstrong, MSc
    Presented at: Microbiology Week Virtual Event Series 2022
    Sponsored By: Jumpcode Genomics
    5
    AUG 16, 2022 | 11:00 AM
    How to Leverage Laser Capture Microdissection Technology for Advanced Oncology Research
    How to Leverage Laser Capture Microdissection Technology for Advanced...
    Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
    Speaker: Katherine Walter
    Sponsored By: Thermo Fisher Scientific
    12
    AUG 10, 2022 | 10:00 AM
    Avoiding hurdles in molecular assay development
    Avoiding hurdles in molecular assay development
    Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
    Speaker: Naeem Nathoo
    Sponsored By: Thermo Fisher Scientific
    16
    JUL 27, 2022 | 9:00 AM
    Accelerating strain development process for CDMO and CRO companies
    Accelerating strain development process for CDMO and CRO companies
    Date: July 27, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Manufacturing of proteins, mRNA or small molecules requires comprehensive strain engineering approaches to increase yield...
    Speaker: Eric Abbate
    Sponsored By: Inscripta
    8 1
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